Concentrations of fluoroquinolones, which are used in the treatment of many bacterial infections, should be monitored in biological fluids as they exhibit concentration‐dependent bactericidal activity. In this study, a liquid chromatography method for the determination of levofloxacin, ciprofloxacin, moxifloxacin and gemifloxacin in human urine and plasma was developed for the first time. The efficiency of five different columns for the separation of these fluoroquinolones was compared. Experimental parameters that affect the separation, such as percentage of organic solvent, pH, temperature, gradient shape and detector wavelength, were optimized by a step‐by‐step approach. Using a pentafluorophenyl core–shell column (100 × 4.6 mm, 2.7 μm), the separation of four analytes was accomplished in <7.5 min. The developed method was validated for the determination of analytes in both urine and plasma with respect to sensitivity, specificity, linearity (r ≥ 0.9989), recovery (79.46–102.69%), accuracy, precision and stability (85.79–111.07%). The intra‐ and inter‐day accuracies were within 89.55–111.94% with relative standard deviations of 0.35–8.05%. The feasibility of method was demonstrated by analyzing urine and plasma samples of patients orally receiving levofloxacin, ciprofloxacin or moxifloxacin. The developed method is suitable for therapeutic drug monitoring of these fluoroquinolones and can be applied to pharmacokinetic and toxicological studies.

中文翻译：

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HPLC-FLD-DAD五氟苯基核壳柱用于尿液和血浆中左氧氟沙星，环丙沙星，莫西沙星和吉非沙星的测定：在药物监测中的应用。

用于治疗许多细菌感染的氟喹诺酮类药物的浓度应在生物液体中进行监测，因为它们表现出浓度依赖性的杀菌活性。本研究首次开发了一种液相色谱法测定人尿和血浆中的左氧氟沙星，环丙沙星，莫西沙星和吉西沙星。比较了五个不同色谱柱分离这些氟喹诺酮的效率。通过分步方法优化了影响分离的实验参数，例如有机溶剂的百分比，pH，温度，梯度形状和检测器波长。使用五氟苯基核壳色谱柱（100×4.6 mm，2.7μm），可以在<7.5分钟内完成四种分析物的分离。ř  ≥0.9989），恢复（79.46-102.69％），准确度，精度和稳定度（85.79-111.07％）。日内和日间准确度在89.55–111.94％之内，相对标准偏差为0.35–8.05％。通过分析口服左氧氟沙星，环丙沙星或莫西沙星患者的尿液和血浆样本，证明了该方法的可行性。所开发的方法适用于这些氟喹诺酮类药物的治疗性药物监测，并可用于药代动力学和毒理学研究。