Calcium phosphates, due to their similarity to the inorganic fraction of mineralized tissues, are of great importance in treatment of bone defects. In order to improve the biological activity of hydroxyapatite (HAP), its fluoride-substituted modification (FAP) was synthesized using the sol-gel method and calcined at three different temperatures in the range of 800–1200 °C. Physicochemical and biological properties were evaluated to indicate which material would support bone regeneration the best. X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray spectrometry (EDS), and Fourier transform infrared spectroscopy (FTIR) revealed that fluoride ions were incorporated into the apatite lattice structure. In studies it was found that fluorapatite sintered at the highest temperature had the lowest porosity, no internal pores and the highest density. In vitro ion reactivity assessments showed that during the 28-day immersion of the samples in the simulated body fluid, the uptake of calcium and phosphorus ions was inversely correlated to the calcination temperature. All tested materials were non-toxic since the cytotoxicity MTT assay demonstrated that the viability of preosteoblast cells incubated with sample extracts was high. Fluorapatite sintered at 800 °C was determined to be of optimal porosity and fluoride release capacity and then used in cell proliferation studies. The results showed that it significantly shortened the doubling time and thus enhanced the proliferation of osteogenic cells, as compared to the fluoride solutions and control group. Therefore, this material is proposed for the use in orthopedic applications and bone tissue engineering.

由于磷酸钙与矿化组织的无机部分相似，因此在治疗骨缺损中非常重要。为了提高羟磷灰石（HAP）的生物活性，使用溶胶-凝胶法合成了其氟化物取代的修饰物（FAP），并在800-1200°C的三种不同温度下煅烧。对理化和生物学特性进行了评估，以表明哪种材料将最好地支持骨骼再生。X射线衍射（XRD），具有能量色散X射线光谱法（EDS）的扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）表明，氟离子被掺入磷灰石晶格结构中。在研究中，发现在最高温度下烧结的氟磷灰石的孔隙率最低，体外离子反应性评估显示，在将样品浸入模拟体液中28天期间，钙和磷离子的吸收与煅烧温度成反比。所有测试材料均无毒，因为细胞毒性MTT分析表明与样品提取物一起孵育的成骨前细胞的活力很高。确定在800°C烧结的氟磷灰石具有最佳的孔隙率和氟化物释放能力，然后用于细胞增殖研究。结果表明，与氟化物溶液和对照组相比，它显着缩短了成倍的时间，从而增强了成骨细胞的增殖。因此，建议将该材料用于骨科应用和骨组织工程。