Biological conversion of lignocellulosic biomass requires high solids concentration for economical recovery of end products. Clostridium thermocellum fermentations were conducted at various concentrations of switchgrass. Significant reduction of carbohydrate solubilization has been observed for switchgrass with increasing solids concentration. Tests were conducted to understand the cause of inhibition at high solids concentration. Avicel hydrolysis by cell-free C. thermocellum cellulase or C. thermocellum fermentation on cellobiose, supplemented with supernatant or solids from C. thermocellum culture on switchgrass, revealed that inhibition was from the supernatant but not from the solids. Culture of C. thermocellum on Avicel, supplemented with supernatant from C. thermocellum monoculture or mixed consortia, indicated that Avicel solubilization was reduced by almost two-fold with the monoculture supernatant while no significant reduction was observed for the mixed consortia supernatant. Adding 8 kg/m³ commercial xylooligomers during C. thermocellum culture on Avicel reduced Avicel consumption up to 90%. Simultaneous consumption of xylooligomers was suggested for industrial application of C. thermocellum culture on high solids concentration.

木质纤维素生物质的生物转化需要高固体浓度才能经济地回收最终产品。在各种浓度的柳枝。中进行了热纤梭菌发酵。随着固形物浓度的增加，柳枝switch的碳水化合物增溶作用明显降低。进行测试以了解在高固体浓度下抑制的原因。通过在纤维二糖上无细胞的热纤梭菌纤维素酶或热纤梭菌发酵进行的Avicel水解，补充了柳枝switch在热纤梭菌培养物中的上清液或固体，发现抑制作用来自上清液，而不是来自固体。热纤梭菌的培养补充了来自热纤梭菌单培养或混合财团的上清液的Avicel的结果表明，单培养上清液使Avicel的溶解减少了几乎两倍，而混合财团的上清液没有观察到明显的减少。在Avicel上进行热纤梭状芽孢杆菌培养时，添加8 kg / m ³商业木寡聚体可将Avicel的消耗量降低90％。建议同时消耗木糖寡聚体以用于在高固体浓度下工业化热纤梭菌培养的工业应用。