The aim of this experiment was to evaluate the effects of bioactive peptides derived from enzymatic hydrolysis of cottonseed meal (CSBP) compared with zinc bacitracin, as an antibiotic growth promoter (AGP), on productive traits, serum lipid profile, and ileal microbial population in broiler chickens. A total number of 240-day-old broiler chicks (Ross 308) were allocated into 4 treatments, replicated 5 times based on a completely randomized design. The dietary treatments included a basal diet serving as control group, basal diet + 40 mg/kg zinc bacitracin as AGP group, and the basal diet supplemented with 15 or 20 g/kg CSBP substituting equal quantity of maize and soybean meal. Performance traits, including daily weight gain, feed intake, feed conversion ratio (FCR), and livability were recorded. At the end of the study, serum lipid parameters, ileal microbial population, and economical indices were determined. The results indicated that feed intake and FCR increased (P < 0.05) in birds receiving 20 g/kg CSBP over the entire period (1–35 days), but there was no significant effect of CSBP on body weight, although numerically higher than the control group. However, the antibiotic group showed a significant increase (P < 0.05) in body weight and feed intake. Livability, European Production Efficiency Factor (EPEF), and European Broiler Index (EBI) significantly improved in broiler chickens fed antibiotic and 15 g/kg CSBP supplement (P < 0.05). Adding 20 g/kg of CSBP to the diet significantly increased serum triglycerides and decreased low-density lipoproteins (LDL) compared to the control and antibiotic groups on day 35. The relative weight of abdominal fat and LDL to HDL ratio were significantly lower for CSBP and antibiotic treatments than the control group (P < 0.05). Supplementation of antibiotic and both graded levels of CSBP decreased the ileum population of Escherichia coli (P < 0.05). The current findings suggest that including CSBP in broiler diets may benefit production through improving growth rate of broilers and balancing gut microbiota population. In addition, CSBP could be considered as a potential alternative to antibiotics in an AGP free production system.

本实验的目的是评估棉籽粕（CSBP）与杆菌肽锌作为抗生素生长促进剂（AGP）相比，酶水解产生的生物活性肽对生产性状，血脂和回肠微生物种群的影响。肉鸡。将总数为240天的肉仔鸡（罗斯308）分配到4种处理中，根据完全随机的设计重复5次。饮食治疗包括：以基础饮食为对照组，以基础饮食+ 40 mg / kg杆菌肽锌作为AGP组，并在基础饮食中补充15或20 g / kg CSBP替代等量的玉米和豆粕。记录性能特征，包括日增重，采食量，饲料转化率（FCR）和宜居性。在研究结束时，血清脂质参数，回肠微生物种群，并确定经济指标。结果表明，在整个时期（1-35天）内，接受20 g / kg CSBP的家禽的采食量和FCR均增加（P <0.05），但CSBP对体重没有显着影响，尽管其数值高于控制组。但是，抗生素组的体重和采食量显着增加（P <0.05）。在饲喂抗生素和15 g / kg CSBP补充剂的肉鸡中，存活率，欧洲生产效率因子（EPEF）和欧洲肉鸡指数（EBI）显着提高（P <0.05）。与第35天的对照组和抗生素组相比，在饮食中添加20 g / kg的CSBP显着增加了血清甘油三酸酯并降低了低密度脂蛋白（LDL）。CSBP和抗生素治疗组的腹部脂肪相对重量和LDL / HDL比值显着低于对照组（P <0.05）。补充抗生素和CSBP的分级水平均降低了回肠的回肠种群。大肠杆菌（P <0.05）。当前的研究结果表明，在肉鸡日粮中添加CSBP可能会通过提高肉鸡的生长速度和平衡肠道菌群而有益于生产。此外，在无AGP的生产系统中，CSBP可以被视为抗生素的潜在替代品。