Lecythis pisonis Cambess, popularly known as sapucaia, has great economic and socio-environmental potential. The objective of this study was to evaluate the establishment and in vitro morphogenesis of L. pisonis under the effect of disinfecting agents, plant growth regulators, and thermal stress. The study was divided into three experiments: (i) development of the disinfection protocol by testing different concentrations and times of exposure to sodium hypochlorite (NaOCl) and different concentrations and methods of amoxicillin application, (ii) in vitro budding induction by testing different concentrations of 6-benzylaminopurine (BAP) or kinetin (KIN) supplemented to Woody Plant Medium (WPM) and Murashige and Skoog (MS) culture media, and (iii) in vitro formation from plantlets by analyzing different concentrations of indole-3-butyric acid (IBA) with different exposure times to a thermal stress of 40°C. The disinfection of stem segments was effective using 3% NaOCl and 3.0 g L⁻¹ amoxicillin solution. MS culture medium supplemented with 0.25 mg L⁻¹ BAP induced more shoots in vitro. One milligram per liter IBA promoted greater rooting in vitro, and it is not necessary for thermal stress tolerance.

金钱草坎贝斯，俗称sapucaia，具有巨大的经济和社会环境潜力。这项研究的目的是评估在消毒剂，植物生长调节剂和热胁迫的作用下，利索氏乳杆菌的建立和体外形态发生。该研究分为三个实验：（i）通过测试不同浓度和时间的次氯酸钠（NaOCl）暴露时间以及不同浓度和阿莫西林的应用方法开发消毒方案，（ii）通过测试不同浓度的体外萌芽诱导木本植物培养基（WPM）和Murashige and Skoog（MS）培养基中补充的6-苄基氨基嘌呤（BAP）或激动素（KIN），以及（iii）通过分析不同浓度的吲哚-3-丁酸（IBA）在40°C的热应力下具有不同的暴露时间，可以从幼苗中体外形成。使用3％NaOCl和3.0 g L ^-1阿莫西林溶液对茎节进行消毒是有效的。MS培养基补充有0.25毫克的L ^-1 BAP诱导了更多的枝条在体外。每升1毫克IBA促进体外更大的生根，并且对于耐热性没有必要。