Recently, more and more new fungal pathogens have been described as causal agents of grapevine trunk diseases (GTDs), which lead to increasingly significant economic losses in viticulture worldwide. The genus Neofabraea consists of species mainly known as important plant pathogens causing perennial canker and bull’s eye rot, a common postharvest disease of apple (Malus domestica) and pear (Pyrus communis) fruits. Neofabraea kienholzii also causes lesions on pome fruits and canker on woody tissues, but its pathogenicity has not been demonstrated on grapevine yet. In 2015, two strains, identified as N. kienholzii based on ITS sequence data, were isolated from vines showing symptoms of GTDs in Hungary. For an unambiguous taxonomic placement of the isolates, four loci (ITS, LSU, TUB2 and RPB2) were amplified and sequenced. The phylogenetic analysis confirmed that the two isolates represent N. kienholzii. Pathogenicity tests performed on potted grapevines, shoots, and canes confirmed the virulence of these fungi. Their growth and sporulation on different media were also investigated. To the best of our knowledge, this is the first proof of N. kienholzii might cause symptoms on Vitis and might have a role in GTDs.

近来，越来越多的新的真菌病原体被描述为葡萄干病（GTDs）的病原体，这导致了全球葡萄栽培中日益严重的经济损失。Neofabraea属由主要被称为重要植物病原体的物种组成，引起多年生溃疡和牛眼腐烂，这是苹果（Malus domestica）和梨（Pyrus communis）果实的常见收获后病害。Neofabraea kienholzii还会在石榴果上造成损害，并在木质组织上引起溃疡，但是其致病性尚未在葡萄树上得到证实。2015年，鉴定为肯尼霍尔猪笼草的两个菌株根据ITS序列数据，从匈牙利显示GTD症状的葡萄藤中分离出来。为了明确分离的分类，将四个基因座（ITS，LSU，TUB2和RPB2）扩增并测序。系统发育分析证实，这两个分离株代表猪链球菌。对盆栽的葡萄藤，枝条和甘蔗进行的致病性测试证实了这些真菌的毒性。还研究了它们在不同培养基上的生长和孢子形成。据我们所知，这是kienholzii猪的第一个证据，它可能导致葡萄膜炎症状并可能在GTD中起作用。