An investigation employing a detached leaf assay was performed to evaluate the biocontrol efficacy of silver nanoparticles (AgNPs) augmented Calothrix elenkinii against Alternaria alternata infection in tomato. A dose of 50 mg l^− 1 AgNPs was selected, which was based on chlorophyll accumulation (as an index of C. elenkinii growth), microscopic observations and biocidal potential of AgNPs against A. alternata. Higher leaf chlorophyll accumulation and lower endoglucanase activity were recorded in in the AgNPs, C. elenkinii and AgNPs augmented C. elenkinii-treated leaves, compared to pathogen challenge alone. Further, microscopic examination showed that these treatments had an alleviating role towards the damaging effects of A. alternata infection on chloroplasts and leaf structure. PCR based amplification using Internal Transcribed Spacers (ITS)-specific primers revealed no band in the treated samples, illustrating the absence of A. alternata, and the biocontrol efficacy of the treatments. Application of AgNPs, C. elenkinii and AgNPs augmented C. elenkinii imparted a protective effect against A. alternata, by inhibiting the growth of the pathogen in tomato leaves and modulating the growth and enzymatic activities of the host. This method proved valuable for gaining insights into the interplay between cyanobacterium, nanoparticle and pathogen-challenged leaves, and illustrated that AgNPs augmented C. elenkinii has the potential to be scaled up as an effective biocontrol option.

进行了一项采用离体叶片分析的研究，以评估银纳米粒（AgNPs）增强的Calothrix elenkinii对番茄中链格孢的感染。根据叶绿素积累（作为弯曲杆菌生长的指标），显微镜观察和AgNPs对交链土壤杆菌的杀菌潜力，选择了50 mg ^-1 AgNPs剂量。在AgNP，C。elenkinii和AgNPs增强的C.elenkinii中记录到较高的叶绿素积累和较低的内切葡聚糖酶活性。处理的叶子，与单独的病原体攻击相比。此外，显微镜检查表明这些处理对交链土壤杆菌感染对叶绿体和叶结构的破坏作用具有缓解作用。PCR使用内转录间隔区的扩增（ITS） -特异性引物处理的样品中没有发现带，示出了不存在的链格孢，和的治疗的功效生物防治。AgNP，C.elenkinii和增强AgNPs的C.elenkinii的应用赋予了对交链曲霉的保护作用通过抑制番茄叶片中病原体的生长并调节宿主的生长和酶活性来实现。该方法被证明对于深入了解蓝细菌，纳米颗粒和病原体挑战的叶片之间的相互作用具有重要价值，并说明AgNPs增强的C. elenkinii具有扩大规模作为有效的生物防治选择的潜力。