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Stabilized albumin coatings on engineered xenografts for attenuation of acute immune and inflammatory responses.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-06-18 , DOI: 10.1039/d0tb01111h Chao Tao 1 , Wenzhen Zhu 1 , Jabed Iqbal 2 , Chenjie Xu 3 , Dong-An Wang 4
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-06-18 , DOI: 10.1039/d0tb01111h Chao Tao 1 , Wenzhen Zhu 1 , Jabed Iqbal 2 , Chenjie Xu 3 , Dong-An Wang 4
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Xenogeneic grafts are promising candidates for transplantation therapy due to their easily accessible sources. Nevertheless, the immune and inflammatory responses induced by xenografts need to be addressed for clinical use. A novel and facile method was introduced for the attenuation of immune and inflammatory responses by extending the immune evasion potential of albumin to the tissue engineering field and coating albumin, which could passivate biomaterial surfaces, onto xenografts. Albumin was first modified by dopamine to enhance its adhesion on graft surfaces. Porcine chondrocytes derived living hyaline cartilage graft (LhCG) and decellularized LhCG (dLhCG) were applied as xenograft models implanted in the omentum of rats. Both LhCG which contained porcine chondrocytes as well as secreted ECM and dLhCG which was mainly composed of the porcine source ECM showed alleviated immune and inflammatory responses after being coated with albumin at cell, protein and gene levels, respectively. Significantly less inflammatory cells including neutrophils, macrophages and lymphocytes were recruited according to pathological analysis and immunohistochemistry staining with lower gene expression encoding inflammation-related cytokines including MCP-1, IL-6 and IL-1β after employing LhCG and dLhCG with albumin passivation coating.
中文翻译：
工程异种移植物上的稳定白蛋白涂层可减轻急性免疫和炎症反应。
异种移植物由于其易于获得的来源而有望用于移植治疗。然而，由异种移植物诱导的免疫和炎症反应需要针对临床使用。通过将白蛋白的免疫逃逸能力扩展到组织工程领域，并涂覆可钝化生物材料表面的白蛋白到异种移植物上，提出了一种新的简便方法来减弱免疫和炎症反应。白蛋白首先被多巴胺修饰，以增强其在移植物表面的附着力。将猪软骨细胞衍生的活透明软骨移植物（LhCG）和脱细胞的LhCG（dLhCG）用作大鼠网膜中的异种移植模型。含有猪软骨细胞以及分泌的ECM的LhCG和主要由猪源ECM组成的dLhCG都分别在细胞，蛋白质和基因水平上被白蛋白包被后显示出减轻的免疫和炎症反应。根据病理分析和免疫组织化学染色，采用LhCG和dLhCG结合白蛋白钝化涂层的炎症分析相关细胞因子（包括MCP-1，IL-6和IL-1β）具有较低的基因表达，因此募集的炎症细胞（包括中性粒细胞，巨噬细胞和淋巴细胞）明显减少。
更新日期：2020-07-22
中文翻译：
工程异种移植物上的稳定白蛋白涂层可减轻急性免疫和炎症反应。
异种移植物由于其易于获得的来源而有望用于移植治疗。然而，由异种移植物诱导的免疫和炎症反应需要针对临床使用。通过将白蛋白的免疫逃逸能力扩展到组织工程领域，并涂覆可钝化生物材料表面的白蛋白到异种移植物上，提出了一种新的简便方法来减弱免疫和炎症反应。白蛋白首先被多巴胺修饰，以增强其在移植物表面的附着力。将猪软骨细胞衍生的活透明软骨移植物（LhCG）和脱细胞的LhCG（dLhCG）用作大鼠网膜中的异种移植模型。含有猪软骨细胞以及分泌的ECM的LhCG和主要由猪源ECM组成的dLhCG都分别在细胞，蛋白质和基因水平上被白蛋白包被后显示出减轻的免疫和炎症反应。根据病理分析和免疫组织化学染色，采用LhCG和dLhCG结合白蛋白钝化涂层的炎症分析相关细胞因子（包括MCP-1，IL-6和IL-1β）具有较低的基因表达，因此募集的炎症细胞（包括中性粒细胞，巨噬细胞和淋巴细胞）明显减少。
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