The thermal stability of purified acid phosphatase from the germinating seedlings of Coronopus didymus (Jangli halon) was investigated by studying the impact of various thermodynamic parameters [t_1/2, E_d, ΔH° (enthalpy change), ΔG° (free energy change), and ΔS° (entropy change)] of heat treatment in the temperature range of 55–75 °C. The thermal denaturation of acid phosphatase, assessed by loss in activity, was evidently followed by first-order kinetics, which varies with time and yield during the process of denaturation. The half-life of the enzyme was 693 min at 55 °C. The E_d (activation energy of denaturation) was calculated by the Arrhenius plot (30 kcal mol^–1), and the Z-value was 17.3 °C. The various thermodynamic parameters studied were as follows: ΔH°, the change in enthalpy of inactivation, was 121.93 kJ mol^–1 at 55 °C; ΔG°, the change in free energy of inactivation, was 110.65 kJ mol^–1 at 55 °C; and ΔS°, the change in entropy of inactivation, was 34.39 J mol^–1 k^–1 at 55 °C. This suggests that acid phosphatase activity is thermostable to long heat treatment up to 60 °C.

中文翻译：

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研究各种参数对 Coronopus didymus 幼苗酸性磷酸酶活性的影响。

通过研究各种热力学参数[ t _1/2 , E _d , Δ H ° (焓变), Δ G ° (游离能量变化）和ΔS °（熵变）]在55-75°C温度范围内的热处理。通过活性损失评估酸性磷酸酶的热变性明显遵循一级动力学，其在变性过程中随时间和产量而变化。该酶的半衰期在 55 °C 下为 693 分钟。教育_署_（变性活化能）由Arrhenius曲线（30 kcal mol ^–1）计算，Z值为17.3°C。研究的各种热力学参数如下：ΔH °，失活焓的变化，在 55 °C 时为 121.93 kJ mol ^{–1 ；}Δ G °，失活自由能的变化，在 55 °C 时为 110.65 kJ mol ^{–1 ；}Δ S °，失活熵的变化，在 55 °C 时为 34.39 J mol ^–1 k ^{–1 。}这表明酸性磷酸酶活性对高达 60 °C 的长时间热处理是热稳定的。