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Three Host Plant Volatiles, Hexanal, Lauric Acid, and Tetradecane, are Detected by an Antenna-Biased Expressed Odorant Receptor 27 in the Dark Black Chafer Holotrichia parallela.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-06-18 , DOI: 10.1021/acs.jafc.0c00333 Xiao Wang 1 , Shang Wang 1 , Jiankun Yi 1 , Yunshuo Li 1 , Jianan Liu 1 , Jun Wang 1 , Jinghui Xi 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-06-18 , DOI: 10.1021/acs.jafc.0c00333 Xiao Wang 1 , Shang Wang 1 , Jiankun Yi 1 , Yunshuo Li 1 , Jianan Liu 1 , Jun Wang 1 , Jinghui Xi 1
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Insects rely on olfaction to locate their host plants by antennae in complex chemical environments. Odorant receptor (OR) genes are thought to play a crucial role in the process. ORs function together with odorant coreceptors to determine the specificity and sensitivity of olfactory reception. The dark black chafer, Holotrichia parallela Motschulsky (Coleoptera: Scarabaeidae), is a destructive underground pest. To understand the molecular basis of H. parallela olfactory reception, an olfactory-biased expressed odorant receptor HparOR27 and HparOrco (HparOR40) were identified from antennal transcriptome analysis and prediction of the sequence structure. Tissue expression analysis showed that HparOR27 was mainly expressed in adult antennae throughout developmental stages. The functions of HparOR27 were analyzed using the Xenopus laevis oocyte expression system. HparOR27 was broadly responsive to three host plant volatiles, including hexanal, lauric acid, and tetradecane. Electroantennogram tests confirmed that three ligands were electrophysiologically active in antennae of female adults. A Y-tube olfactometer test indicated that hexanal was a repellent for adults of both sexes. Taken together, our data support the identification of odorant receptors and provide a molecular basis for eco-friendly pest control.
中文翻译：
在深黑色金龟子平行线虫中，通过天线偏置的表达异味受体27检测到了三个寄主植物的挥发物，即己醛，月桂酸和十六烷。
昆虫在复杂的化学环境中依靠嗅觉通过触角定位寄主植物。气味受体（OR）基因被认为在这一过程中起着至关重要的作用。OR与气味共受体一起起作用，以确定嗅觉接收的特异性和敏感性。暗黑色金龟子（Holotrichia parallela Motschulsky，鞘翅目：Scarabaeidae）是一种破坏性的地下害虫。了解平行链球菌的分子基础通过触角转录组分析和序列结构预测，鉴定了嗅觉接收，嗅觉偏向的表达气味受体HparOR27和HparOrco（HparOR40）。组织表达分析表明，HparOR27在整个发育阶段主要在成人触角中表达。使用非洲爪蟾（Xenopus laevis）分析了HparOR27的功能卵母细胞表达系统。HparOR27对三种寄主植物挥发物具有广泛的响应性，包括己醛，月桂酸和十四烷。脑电图测试证实，三个配体在成年雌性触角中具有电生理活性。Y型管嗅觉仪测试表明，己醛对男女成人均具有驱避作用。综上所述，我们的数据支持识别气味受体，并为生态友好的害虫防治提供分子基础。
更新日期：2020-07-15
中文翻译：
在深黑色金龟子平行线虫中，通过天线偏置的表达异味受体27检测到了三个寄主植物的挥发物，即己醛，月桂酸和十六烷。
昆虫在复杂的化学环境中依靠嗅觉通过触角定位寄主植物。气味受体（OR）基因被认为在这一过程中起着至关重要的作用。OR与气味共受体一起起作用，以确定嗅觉接收的特异性和敏感性。暗黑色金龟子（Holotrichia parallela Motschulsky，鞘翅目：Scarabaeidae）是一种破坏性的地下害虫。了解平行链球菌的分子基础通过触角转录组分析和序列结构预测，鉴定了嗅觉接收，嗅觉偏向的表达气味受体HparOR27和HparOrco（HparOR40）。组织表达分析表明，HparOR27在整个发育阶段主要在成人触角中表达。使用非洲爪蟾（Xenopus laevis）分析了HparOR27的功能卵母细胞表达系统。HparOR27对三种寄主植物挥发物具有广泛的响应性，包括己醛，月桂酸和十四烷。脑电图测试证实，三个配体在成年雌性触角中具有电生理活性。Y型管嗅觉仪测试表明，己醛对男女成人均具有驱避作用。综上所述，我们的数据支持识别气味受体，并为生态友好的害虫防治提供分子基础。
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