Variation in numbers and functions of cells in fat tissues may affect therapeutic outcomes and adverse events after autologous fat tissue grafting in postmastectomy breast cancer patients; however, the relevant information regarding cellular components is still incomplete. Phenotypic characterization of heterogeneous cell subsets in stromal vascular fraction (SVF) isolated from fat tissues by flow cytometry was also limited to a combination of few molecules. This study, therefore, developed a polychromatic staining panel for an in-depth characterization of freshly isolated SVF and expanded adipose-derived stem cells (ADSC) from the patients. ADSC were found predominant in SVF (~65% of CD45^- cells) with a homogenous phenotype of CD13⁺CD31^-CD34⁺CD45^-CD73⁺CD90⁺CD105^-CD146^- (~94% of total ADSC). Endothelial progenitor cells (EPC) and pericytes were minor (~18% and ~11% of CD45^- cells, respectively) with large heterogeneity. Downregulation of CD34 and upregulation of CD105 in ADSC were profound at passage 3, showing a phenotype similar to the classical mesenchymal stem cells from the bone marrow. Results from this study demonstrated that fat tissue collected from patients contains ADSC with a highly homogenous phenotype. The in vitro culture of these cells maintained their homogeneity with modified CD34 and CD105 expression, suggesting the expansion from a single population of ADSC.

中文翻译：

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从乳腺癌患者新鲜分离和体外扩增的人脂肪干细胞上的间充质干细胞标志物表达的广泛表征。

脂肪组织中脂肪组织中细胞数量和功能的变化可能会影响乳腺癌切除术后自体脂肪组织移植后的治疗结果和不良事件；但是，有关细胞成分的相关信息仍然不完整。通过流式细胞术从脂肪组织中分离出的基质血管部分（SVF）中异质细胞亚型的表型表征也仅限于少数分子的组合。因此，本研究开发了一种多色染色板，用于深入表征患者的新鲜分离的SVF和扩增的脂肪干细胞（ADSC）。ADSC被发现主要在SVF（CD45的〜65％^-细胞）与CD13的均匀表型⁺ CD31 ^- CD34 ⁺CD45 ^- CD73 ⁺ CD90 ⁺ CD105 ^- CD146 ^{- （〜}总ADSC的94％）。内皮祖细胞（EPC）和周细胞是轻微（〜18％和〜CD45的11％^-分别细胞）具有大的异质性。在第3代中，ADSC中CD34的下调和CD105的上调很明显，显示出与来自骨髓的经典间充质干细胞相似的表型。这项研究的结果表明，从患者体内收集的脂肪组织包含具有高度同质表型的ADSC。在体外，这些细胞的培养物保持其均匀性与改性CD34和CD105表达，提示从ADSC的单个群体的膨胀。