Background. Many neurodegenerative diseases such as Alzheimer’s disease are associated with oxidative stress. Therefore, antioxidant therapy has been suggested for the prevention and treatment of neurodegenerative diseases. Objective. We investigated the ability of the antioxidant Antia to exert a protective effect against sporadic Alzheimer’s disease (SAD) induced in mice. Antia is a natural product that is extracted from the edible yamabushitake mushroom, the gotsukora and kothala himbutu plants, diosgenin (an extract from wild yam tubers), and amla (Indian gooseberry) after treatment with MRN-100. Methods. Single intracerebroventricular (ICV) injection of streptozotocin (STZ) (3 mg/kg) was used for induction of SAD in mice. Antia was injected intraperitoneally (i.p.) in 3 doses (25, 50, and 100 mg/kg/day) for 21 days. Neurobehavioral tests were conducted within 24 h after the last day of injection. Afterwards, mice were sacrificed and their hippocampi were rapidly excised, weighed, and homogenized to be used for measuring biochemical parameters. Results. Treatment with Antia significantly improved mice performance in the Morris water maze. In addition, biochemical analysis showed that Antia exerted a protective effect for several compounds, including GSH, MDA, NF-κB, IL-6, TNF-α, and amyloid β. Further studies with western blot showed the protective effect of Antia for the JAK2/STAT3 pathway. Conclusions. Antia exerts a significant protection against cognitive dysfunction induced by ICV-STZ injection. This effect is achieved through targeting of the amyloidogenic, inflammatory, and oxidative stress pathways. The JAK2/STAT3 pathway plays a protective role for neuroinflammatory and neurodegenerative diseases such as SAD.

中文翻译：

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Antia 是一种天然抗氧化产品，通过靶向淀粉样蛋白生成、炎症、自噬和氧化应激途径，减轻链脲佐菌素诱导的散发性阿尔茨海默病小鼠模型中的认知功能障碍。

背景。许多神经退行性疾病，如阿尔茨海默病，都与氧化应激有关。因此，抗氧化疗法被建议用于预防和治疗神经退行性疾病。客观。我们研究了抗氧化剂 Antia 对小鼠诱发的散发性阿尔茨海默病 (SAD) 发挥保护作用的能力。Antia 是从可食用的山药菇、gotsukora 和 kothala himbutu 植物、薯蓣皂苷元（野生山药块茎提取物）和 amla（印度醋栗）中提取的天然产品，经过 MRN-100 处理。方法. 单次脑室内 (ICV) 注射链脲佐菌素 (STZ) (3 mg/kg) 用于诱导小鼠的 SAD。Antia 以 3 个剂量（25、50 和 100 mg/kg/天）腹膜内 (ip) 注射 21 天。在注射最后一天后的 24 小时内进行神经行为测试。之后，处死小鼠，快速切除其海马体，称重并均质化，用于测量生化参数。结果。Antia 治疗显着改善了小鼠在莫里斯水迷宫中的表现。此外，生化分析表明 Antia 对多种化合物具有保护作用，包括 GSH、MDA、NF- κ B、IL-6、TNF- α和淀粉样蛋白β. 进一步的蛋白质印迹研究表明 Antia 对 JAK2/STAT3 通路具有保护作用。结论。Antia 对 ICV-STZ 注射引起的认知功能障碍具有显着的保护作用。这种效果是通过靶向淀粉样蛋白生成、炎症和氧化应激途径来实现的。JAK2/STAT3 通路对神经炎症和神经退行性疾病如 SAD 起保护作用。