Noncovalent Protein Glycosylation Strategy via In Situ Nanoencapsulation,ACS Applied Bio Materials

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Noncovalent Protein Glycosylation Strategy via In Situ Nanoencapsulation
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-17 , DOI: 10.1021/acsabm.0c00486
Xianru Han ₁ , Shuo Qin ₁ , Xianyan Qin ₁ , Tao Gong ₁ , Zhi-Rong Zhang ₁ , Yao Fu ₁

Affiliation

An efficient strategy for noncovalent protein glycosylation has been developed by in situ saccharide-based nanoencapsulation. The positive monomer dimethylaminoethyl methacrylate is enriched on the protein surface via electrostatic adhesion, enabling free radical polymerization to generate nanocapsules with saccharides on the surface of the protein complex. Comparable to the glycosylation of native proteins, the d-fructose coated polymeric shell layer significantly enhanced the stability of proteins, rendering proteins with cell selectivity and tumor-specific distribution in MCF-7 breast tumor-bearing mice xenografts. Also, glycosylation improved the shelf life of therapeutic proteins which would further benefit manufacturing and facilitate transportation.

中文翻译：

通过原位纳米包封的非共价蛋白糖基化策略

通过原位糖基纳米包封开发了一种有效的非共价蛋白质糖基化策略。正单体甲基丙烯酸二甲氨基乙酯通过静电粘附富集在蛋白质表面，使自由基聚合在蛋白质复合物表面产生含有糖类的纳米胶囊。与天然蛋白质的糖基化相比，d-果糖涂层的聚合物壳层显着增强了蛋白质的稳定性，使蛋白质在携带 MCF-7 乳腺肿瘤的小鼠异种移植物中具有细胞选择性和肿瘤特异性分布。此外，糖基化提高了治疗性蛋白质的保质期，这将进一步有利于制造并促进运输。

更新日期：2020-07-20

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