Abstract

The study was designed to investigate the effect of chemokine CXCL14 on in vitro angiogenesis of human hepatocellular carcinoma (HCC) cells. CXCL14 mRNA expression in HCC tissue samples and adjacent tissue samples was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). CXCL14 mRNA and protein expression in human normal hepatocyte HL-7702 and HCC cell line HepG2 were detected by qRT-PCR and western blot. In HepG2 cell line, the expression of vascular endothelial growth factor (VEGF) was detected by enzyme-linked immunosorbent assay method, cell viability was detected by CCK-8, cell proliferation was detected by colony formation assay, and cell migration as well as invasion ability was detected by Transwell assay. Moreover, human umbilical vein endothelial cell (HUVEC) tube formation assay was carried out to determine the cell ability of angiogenesis. Results showed that the overexpression of CXCL14 could inhibit angiogenesis, proliferation, migration, and invasion abilities of HCC cells.

• Highlights

•  CXCL14 is lowly expressed in hepatocellular carcinoma tissues and cells.

•  CXCL14 overexpression inhibits the angiogenesis of hepatocellular carcinoma cells.

•  CXCL14 overexpression inhibits proliferation, invasion, and migration of hepatocellular carcinoma cells.

摘要

该研究旨在研究趋化因子 CXCL14 对体外人肝细胞癌（HCC）细胞的血管生成。通过定量逆转录聚合酶链反应（qRT-PCR）检测HCC组织样本和邻近组织样本中CXCL14 mRNA的表达。通过qRT-PCR和蛋白质印迹检测人正常肝细胞HL-7702和HCC细胞系HepG2中CXCL14 mRNA和蛋白的表达。酶联免疫吸附法检测HepG2细胞系血管内皮生长因子（VEGF）的表达，CCK-8法检测细胞活力，集落形成法检测细胞增殖，细胞迁移和侵袭。 Transwell法检测能力。此外，进行了人脐静脉内皮细胞（HUVEC）管形成测定以确定血管生成的细胞能力。

• 强调

•  CXCL14 在肝细胞癌组织和细胞中低表达。

•  CXCL14 过表达抑制肝细胞癌细胞的血管生成。

•  CXCL14 过表达抑制肝细胞癌细胞的增殖、侵袭和迁移。