ABSTRACT

DP23211 maize was genetically modified (GM) to express DvSSJ1 double-stranded RNA and the IPD072Aa protein for control of corn rootworm (Diabrotica spp.). DP23211 maize also expresses the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide, and the phosphomannose isomerase (PMI) protein that was used as a selectable marker. A multi-location field trial was conducted during the 2018 growing season at 12 sites selected to be representative of the major maize-growing regions of the U.S. and Canada. Standard agronomic endpoints as well as compositional analytes from grain and forage (e.g., proximates, fibers, minerals, amino acids, fatty acids, vitamins, anti-nutrients, secondary metabolites) were evaluated and compared to non-GM near-isoline control maize (control maize) and non-GM commercial maize (reference maize). A small number of agronomic endpoints were statistically significant compared to the control maize, but were not considered to be biologically relevant when adjusted using the false discovery rate method (FDR) or when compared to the range of natural variation established from in-study reference maize. A small number of composition analytes were statistically significant compared to the control maize. These analytes were not statistically significant when adjusted using FDR, and all analyte values fell within the range of natural variation established from in-study reference range, literature range or tolerance interval, indicating that the composition of DP23211 maize grain and forage is substantially equivalent to conventional maize represented by non-GM near-isoline control maize and non-GM commercial maize.

 抽象的

DP23211 玉米经过基因改造 (GM)，表达 DvSSJ1 双链 RNA 和 IPD072Aa 蛋白，用于控制玉米根虫（根虫属）。 DP23211 玉米还表达草丁膦乙酰转移酶 (PAT) 蛋白以耐受草铵膦除草剂，以及用作选择标记的磷酸甘露糖异构酶 (PMI) 蛋白。 2018 年生长季期间，在代表美国和加拿大主要玉米种植区的 12 个地点进行了多地点田间试验。对标准农艺终点以及谷物和饲料的成分分析物（例如，原品、纤维、矿物质、氨基酸、脂肪酸、维生素、抗营养物、次生代谢物）进行了评估，并与非转基因近等系对照玉米进行比较。对照玉米）和非转基因商业玉米（参考玉米）。与对照玉米相比，少数农艺终点具有统计显着性，但在使用错误发现率方法 (FDR) 调整或与研究中参考玉米建立的自然变异范围进行比较时，不被认为具有生物学相关性。与对照玉米相比，少数成分分析物具有统计显着性。当使用 FDR 调整时，这些分析物不具有统计显着性，并且所有分析物值均落在根据研究中参考范围、文献范围或公差区间确定的自然变异范围内，表明 DP23211 玉米籽粒和饲料的成分基本上相当于传统玉米以非转基因近等系对照玉米和非转基因商品玉米为代表。