ABSTRACT

Food adulteration has a direct impact on public health, religious faith, fair-trades, and wildlife. In the present study, a reliable and sensitive assay has been developed for verifying meat adulteration in food chain. The multiplex PCR system was optimised for identification of chicken, cow/buffalo, sheep/goat, horse/donkey, pork, and dog DNAs in a single reaction mixture simultaneously. The primers were designed using 12 S rRNA gene sequences with fragment size in the range of 113 bp to 800 bp, which can be easily visualised on agarose gel electrophoresis making the technique economical. After validation of accuracy, specificity, and sensitivity, commercially available meat products (n = 190) were screened, comprising both raw and cooked meat samples. The results demonstrated a high rate of adulteration (54.5%) in meat products. The technique developed here can be easily used for screening of different meat products for export and import purposes as well as for food inspection and livestock diagnostic laboratories.

摘要

食品掺假对公共卫生，宗教信仰，公平贸易和野生生物有直接影响。在本研究中，已经开发出一种可靠且灵敏的测定方法，用于验证食物链中的肉类掺假。优化了多重PCR系统，可同时鉴定单一反应混合物中的鸡肉，牛/水牛，绵羊/山羊，马/驴，猪肉和狗DNA。使用12 S rRNA基因序列设计引物，其片段大小在113 bp至800 bp范围内，可在琼脂糖凝胶电泳上轻松观察到，从而使该技术经济。经过验证的准确性，特异性和敏感性后，市售肉类产品（n= 190）进行了筛分，包括生肉和熟肉样品。结果表明，肉制品中的掺假率很高（54.5％）。此处开发的技术可以轻松用于出口和进口目的的不同肉类产品的筛选，以及食品检验和牲畜诊断实验室。