ABSTRACT

Cell blocks and fine needle aspirations can be used for cytopathological diagnosis. Conventional fine needle aspiration smears provide limited material for diagnosis. The cell block technique provides more tissue, which improves diagnostic accuracy. We compared a modified cell block cytology to fine needle aspiration for providing optimal preservation of histochemical and immunocytochemical properties. We used 30 fine needle aspirates from oral lesions in two groups: group 1, fine needle aspiration cytology; group 2, cell block cytology. Smears of fine needle aspirates were stained with Papanicolaou. For the modified cell block technique, aspirated material was centrifuged to create a cell pellet, which then was fixed with Nathan alcohol formalin substitute. After routine histopathological processing, cell pellets were embedded in paraffin, then sectioned and stained with hematoxylin and eosin. Sections were compared to Papanicolaou stained smears of fine needle samples. Cellular morphology and staining quality of modified cell block samples were superior to fine needle aspiration cytology; both methods exhibited distinct nuclear morphology. Modified cell blocks provide excellent cytopathologic features compared to fine needle aspiration cytology.

摘要

细胞块和细针穿刺可用于细胞病理学诊断。传统的细针抽吸涂片提供的诊断材料有限。细胞块技术提供了更多的组织，从而提高了诊断的准确性。我们将改良的细胞块细胞学与细针抽吸进行了比较，以提供组织化学和免疫细胞化学特性的最佳保存。我们在两组中使用了 30 次来自口腔病变的细针抽吸物：第一组，细针抽吸细胞学；第 2 组，细胞块细胞学。细针抽吸物涂片被巴氏染色。对于改良的细胞块技术，将吸出的材料离心以产生细胞沉淀，然后用内森醇福尔马林替代品固定。常规组织病理学处理后，将细胞沉淀包埋在石蜡中，然后切片并用苏木精和伊红染色。将切片与细针样品的巴氏染色涂片进行比较。修饰细胞块样品的细胞形态和染色质量优于细针抽吸细胞学；两种方法都表现出不同的核形态。与细针抽吸细胞学相比，改良的细胞块提供了出色的细胞病理学特征。