In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC–MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. However, the fragmentation masses with m/z of 320, 305, 277 and 220 specific to the CPT could be identified only for the OmF3 and OmF4. These CPT producing fungi were further identified as Meyerozyma sp. OmF3 and Talaromyces sp. OmF4. The cultures of these two fungi were then supplemented with nanoparticles and analyzed for the quantitative enhancement of CPT production by LC–MS/MS. From the result, Meyerozyma sp. OmF3 was found to produce 947.3 ± 12.66 μg/L CPT, when supplemented with 1 μg/mL zinc oxide nanoparticles and the same for uninduced parental strain OmF3 was only 1.77 ± 0.13 μg/L. At the same time, Talaromyces sp. OmF4 showed the highest production of 28.97 ± 0.37 μg/L of CPT when cultured with 10 μg/mL silver nanoparticles and the same for uninduced strain was 1.19 ± 0.24 μg/L. The observed quantitative enhancement of fungal CPT production is highly interesting as it is a rapid and cost effective method. The study is remarkable due to the identification of novel fungal sources for CPT production and its enhancement by nanoparticle supplementation.

中文翻译：

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基于纳米激发剂的增强从 Ophiorrhiza mungos 分离的真菌中的喜树碱产量。

在这项研究中，通过高效液相色谱 (HPLC) 筛选了从蛇根草中分离出的内生真菌的喜树碱 (CPT) 生物合成潜力。在筛选的 16 种真菌中，确定了 OmF3、OmF4 和 OmF6 可合成 CPT。进一步的 LC-MS 分析还显示，从 OmF3、OmF4 和 OmF6 中提取的 CPT 特定m/z为 349。然而，CPT 特有的m/z为 320、305、277 和 220的碎裂质量只能针对 OmF3 和 OmF4 进行鉴定。这些产生 CPT 的真菌被进一步鉴定为Meyerozyma sp。OmF3 和踝节菌sp. OMF4。然后用纳米颗粒补充这两种真菌的培养物，并通过 LC-MS/MS 分析 CPT 生产的定量增强。从结果来看，Meyerozyma sp。发现 OmF3 产生 947.3 ± 12.66 μg/L 的 CPT，当补充 1 μg/mL 氧化锌纳米颗粒时，未诱导的亲本菌株 OmF3 的浓度仅为 1.77 ± 0.13 μg/L。同时，Talaromycessp. 当与 10 μg/mL 银纳米粒子一起培养时，OmF4 显示出最高的 CPT 产量为 28.97 ± 0.37 μg/L，对于未诱导的菌株，同样为 1.19 ± 0.24 μg/L。观察到的真菌 CPT 生产的定量增强非常有趣，因为它是一种快速且具有成本效益的方法。由于确定了用于 CPT 生产的新型真菌来源并通过纳米颗粒补充对其进行了增强，因此该研究非常引人注目。