Whole‐body clearing of beetles by successive treatment with hydrogen peroxide and CUBIC reagents,Entomological Science

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Whole‐body clearing of beetles by successive treatment with hydrogen peroxide and CUBIC reagents
Entomological Science ( IF 0.7 ) Pub Date : 2020-06-18 , DOI: 10.1111/ens.12427
Monto Kuroda ₁ , Shinya Kuroda ₂

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Internal tissues of multicellular organisms cannot directly be seen because they contain pigments. For this reason, whole‐body clearing methods have been developed and applied to mammals such as mice. Insects such as beetles, however, cannot be cleared by the mammalian method because of pigments such as melanin in their exoskeletons. In this study, we tried to develop a whole‐body clearing method for large beetles. We first bleached the exoskeleton using a hydrogen peroxide treatment, and applied advanced Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) reagents to make the internal tissues transparent. The combined method of hydrogen peroxide and advanced CUBIC allowed us to successfully undertake whole‐body clearing of large beetles.

中文翻译：

用过氧化氢和 CUBIC 试剂连续处理甲虫的全身清除

不能直接看到多细胞生物的内部组织，因为它们含有色素。出于这个原因，已经开发出全身清除方法并应用于小鼠等哺乳动物。然而，甲虫等昆虫无法通过哺乳动物的方法清除，因为它们的外骨骼中含有黑色素等色素。在这项研究中，我们尝试开发一种针对大型甲虫的全身清除方法。我们首先使用过氧化氢处理漂白外骨骼，并应用先进的清晰、无障碍大脑/身体成像鸡尾酒和计算分析 (CUBIC) 试剂使内部组织透明。过氧化氢和先进的 CUBIC 相结合的方法使我们能够成功地对大型甲虫进行全身清除。

更新日期：2020-06-18

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