The present study was focused on exploring the presence of active compounds in Origanum majorana essential oil (OmEO), and its various knock‐down effects against the rice moth, Corcyra cephalonica. GC–MS analysis detected the existence of major compounds such as monoterpenes, cis‐β‐terpineol and terpinen‐4‐ol with the total proportion of 52.16%. Fumigant toxicity against adult and larvae was calculated with an LC₅₀ value of 11.31 and 49.83 μL/L air, respectively. The contact toxicity against adult, pupa, larvae and eggs was observed with LC₅₀ value 2.54, 0.95, 2.78, and 0.49 μL/L, respectively. Furthermore, the influential repellent behavior against adults has been observed. Acetylesterase (AChE) inhibition activity of OmEO was observed against adult and larvae of C. cephalonica with an IC₅₀ value of 35.89 and 118.54 μL/mL, respectively. Moreover, computational docking study revealed the binding affinity of Cis‐β‐terpineol and terpinen‐4‐ol towards the active binding sites of AChE. On the other hand, Fluorescence‐assisted cytometry and comet assay confirmed the cytotoxic and genotoxic effect of OmEO at various concentrations on C. cephalonica. Altogether, the results showed the knock‐down effect of OmEO against C. cephalonica, and it could be a potential biocontrol measure against the stored product pest.

中文翻译：

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牛至精油作为抗胆碱药的杀虫活性

本研究集中在探索活性化合物的存在甘牛至精油（OmEO），及其各种击倒靠在米蛾，效果米蛾cephalonica。GC-MS分析检测到主要化合物的存在，例如单萜类，顺式-β-松油醇和萜品-4-醇，总比例为52.16％。熏蒸剂对成年和幼虫的毒性分别通过空气的LC ₅₀值为11.31和49.83μL/ L进行计算。观察到对成年，4，幼虫和卵的接触毒性，LC ₅₀值分别为2.54、0.95、2.78和0.49μL/ L。此外，已经观察到针对成年人的有影响力的驱避行为。乙酰酯酶（乙酰胆碱酯酶）的抑制活性OmEO观察到对成人和幼虫C. cephalonica与IC ₅₀分别35.89和118.54μL/毫升的值，。此外，计算对接研究揭示了顺式β-松油醇和松油烯-4-醇对AChE活性结合位点的结合亲和力。在另一方面，荧光辅助术和彗星试验证实的细胞毒性和遗传毒性作用OmEO各种浓度上C. cephalonica。总之，结果显示的击倒效果OmEO对C. cephalonica ，故能对存储产品害虫潜在的生物防治措施。