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DNA methylation establishment of CpG islands near maternally imprinted genes on chromosome 7 during mouse oocyte growth.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-06-17 , DOI: 10.1002/mrd.23395 Qian-Nan Li 1, 2 , Jun-Yu Ma 2 , Wen-Bo Liu 1 , Tie-Gang Meng 1 , Feng Wang 1 , Yi Hou 1 , Heide Schatten 3 , Qing-Yuan Sun 1, 4 , Xiang-Hong Ou 2
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-06-17 , DOI: 10.1002/mrd.23395 Qian-Nan Li 1, 2 , Jun-Yu Ma 2 , Wen-Bo Liu 1 , Tie-Gang Meng 1 , Feng Wang 1 , Yi Hou 1 , Heide Schatten 3 , Qing-Yuan Sun 1, 4 , Xiang-Hong Ou 2
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The genome methylation is globally erased in early fetal germ cells, and it is gradually re‐established during gametogenesis. The expression of some imprinted genes is regulated by the methylation status of CpG islands, while the exact time of DNA methylation establishment near maternal imprinted genes during oocyte growth is not well known. Here, growing oocytes were divided into three groups based on follicle diameters including the S‐group (60–100 μm), M‐group (100–140 μm), and L‐group (140–180 μm). The fully grown germinal vesicle (GV)‐stage and metaphase II (M2)‐stage mature oocytes were also collected. These oocytes were used for single‐cell bisulfite sequencing to detect the methylation status of CpG islands near imprinted genes on chromosome 7. The results showed that the CpG islands near Ndn , Magel2 , Mkrn3 , Peg12 , and Igf2 were completely unmethylated, but those of Peg3 , Snrpn , and Kcnq1ot1 were hypermethylated in MII‐stage oocytes. The methylation of CpG islands near different maternal imprinted genes occurred asynchronously, being completed in later‐stage growing oocytes, fully grown GV oocytes, and mature MII‐stage oocytes, respectively. These results show that CpG islands near some maternally imprinted genes are not necessarily methylated, and that the establishment of methylation of other maternally imprinted genes is completed at different stages of oocyte growth, providing a novel understanding of the establishment of maternally imprinted genes in oocytes.
中文翻译：
小鼠卵母细胞生长过程中 7 号染色体上母系印记基因附近 CpG 岛的 DNA 甲基化建立。
早期胎儿生殖细胞中的基因组甲基化被全面消除，并在配子发生过程中逐渐重新建立。一些印记基因的表达受到CpG岛甲基化状态的调节，而在卵母细胞生长过程中母体印记基因附近DNA甲基化建立的确切时间尚不清楚。在这里，生长的卵母细胞根据卵泡直径分为三组，包括S组（60-100μm）、M组（100-140μm）和L组（140-180μm）。还收集了完全生长的生殖囊泡 (GV) 阶段和中期 II (M2) 阶段的成熟卵母细胞。利用这些卵母细胞进行单细胞亚硫酸氢盐测序，检测7号染色体印记基因附近CpG岛的甲基化状态。结果显示，Ndn、Magel2、Mkrn3、Peg12和Igf2附近的CpG岛完全未甲基化，但Peg3、Snrpn和Kcnq1ot1在 MII 期卵母细胞中高度甲基化。不同母体印记基因附近的CpG岛甲基化是异步发生的，分别在生长后期卵母细胞、完全生长的GV卵母细胞和成熟MII阶段卵母细胞中完成。这些结果表明，一些母性印记基因附近的CpG岛不一定是甲基化的，而其他母性印记基因的甲基化的建立是在卵母细胞生长的不同阶段完成的，为卵母细胞中母性印记基因的建立提供了新的认识。
更新日期：2020-07-28
中文翻译：
小鼠卵母细胞生长过程中 7 号染色体上母系印记基因附近 CpG 岛的 DNA 甲基化建立。
早期胎儿生殖细胞中的基因组甲基化被全面消除，并在配子发生过程中逐渐重新建立。一些印记基因的表达受到CpG岛甲基化状态的调节，而在卵母细胞生长过程中母体印记基因附近DNA甲基化建立的确切时间尚不清楚。在这里，生长的卵母细胞根据卵泡直径分为三组，包括S组（60-100μm）、M组（100-140μm）和L组（140-180μm）。还收集了完全生长的生殖囊泡 (GV) 阶段和中期 II (M2) 阶段的成熟卵母细胞。利用这些卵母细胞进行单细胞亚硫酸氢盐测序，检测7号染色体印记基因附近CpG岛的甲基化状态。结果显示，Ndn、Magel2、Mkrn3、Peg12和Igf2附近的CpG岛完全未甲基化，但Peg3、Snrpn和Kcnq1ot1在 MII 期卵母细胞中高度甲基化。不同母体印记基因附近的CpG岛甲基化是异步发生的，分别在生长后期卵母细胞、完全生长的GV卵母细胞和成熟MII阶段卵母细胞中完成。这些结果表明，一些母性印记基因附近的CpG岛不一定是甲基化的，而其他母性印记基因的甲基化的建立是在卵母细胞生长的不同阶段完成的，为卵母细胞中母性印记基因的建立提供了新的认识。
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