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Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state.
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-07-06 , DOI: 10.1111/omi.12303 Arancha Llama-Palacios 1, 2 , Oksana Potupa 1 , María C Sánchez 1, 2 , Elena Figuero 2 , David Herrera 2 , Mariano Sanz 2
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-07-06 , DOI: 10.1111/omi.12303 Arancha Llama-Palacios 1, 2 , Oksana Potupa 1 , María C Sánchez 1, 2 , Elena Figuero 2 , David Herrera 2 , Mariano Sanz 2
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Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression of F. nucleatum when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D‐DIGE™ system. In all, 68 proteins were differentially expressed during biofilm growth (1.5‐fold, p < .05), being 20 downexpressed and 31 overexpressed. The repressed proteins belonged to metabolism, biosynthesis and were outer membrane proteins (OMPs); and overexpressed were proteins involved in metabolism, transcription, translation, transport and proteins with unknown function. Also, of the seven enzymes that regulate the synthesis of butyrate, six of them were differentially expressed (overexpressed and downexpressed) when the bacteria were forming biofilms. The enzymatic activities of two of the enzymes in the butyrate pathway were analysed when the bacteria were growing in biofilms or in planktonic growth. All these results confirmed that this metabolic pathway is important in the formation of the biofilm of F. nucleatum and in its pathogenicity, both in the oral cavity and in other locations of the body.
中文翻译：
蛋白质组学分析了生物膜中浮游细菌核的生长与浮游状态的关系。
从人的龈上和龈下牙齿生物膜中分离出核梭状芽胞杆菌，并与牙周炎的病因有关。同样，这种细菌在身体其他部位的严重感染中也起着重要作用。这项研究的目的是使用2D-DIGE™系统进行的蛋白质组分析，研究与浮游状态相比，在生物膜上生长的核镰刀菌的蛋白质差异表达。在生物膜生长过程中，共有68种蛋白质差异表达（1.5倍，p <.05），则有20个表达不足和31个过度表达。抑制的蛋白属于代谢，生物合成，是外膜蛋白（OMPs）。过表达的是参与代谢，转录，翻译，转运和功能未知的蛋白质。同样，在调节丁酸合成的7种酶中，当细菌形成生物膜时，其中6种被差异表达（过表达和下表达）。当细菌在生物膜或浮游生物中生长时，分析了丁酸途径中两种酶的酶活性。所有这些结果证实，该代谢途径在核果镰刀菌生物膜的形成中很重要。 以及在口腔和身体其他部位的致病性。
更新日期：2020-07-06
中文翻译：
蛋白质组学分析了生物膜中浮游细菌核的生长与浮游状态的关系。
从人的龈上和龈下牙齿生物膜中分离出核梭状芽胞杆菌，并与牙周炎的病因有关。同样，这种细菌在身体其他部位的严重感染中也起着重要作用。这项研究的目的是使用2D-DIGE™系统进行的蛋白质组分析，研究与浮游状态相比，在生物膜上生长的核镰刀菌的蛋白质差异表达。在生物膜生长过程中，共有68种蛋白质差异表达（1.5倍，p <.05），则有20个表达不足和31个过度表达。抑制的蛋白属于代谢，生物合成，是外膜蛋白（OMPs）。过表达的是参与代谢，转录，翻译，转运和功能未知的蛋白质。同样，在调节丁酸合成的7种酶中，当细菌形成生物膜时，其中6种被差异表达（过表达和下表达）。当细菌在生物膜或浮游生物中生长时，分析了丁酸途径中两种酶的酶活性。所有这些结果证实，该代谢途径在核果镰刀菌生物膜的形成中很重要。 以及在口腔和身体其他部位的致病性。
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