Vibrios from the Norwegian marine environment: Characterization of associated antibiotic resistance and virulence genes.,MicrobiologyOpen

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Vibrios from the Norwegian marine environment: Characterization of associated antibiotic resistance and virulence genes.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-06-17 , DOI: 10.1002/mbo3.1093
Fredrik Håkonsholm ₁ , Bjørn Tore Lunestad ₁ , Jose Roberto Aguirre Sánchez ₂ , Jaime Martinez-Urtaza ₃ , Nachiket Prakash Marathe ₁ , Cecilie Smith Svanevik ₁

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A total of 116 Vibrio isolates comprising V. alginolyticus (n = 53), V. metschnikovii (n = 38), V. anguillarum (n = 21), V. antiquarius (n = 2), and V. fujianensis (n = 2) were obtained from seawater, fish, or bivalve molluscs from temperate Oceanic and Polar Oceanic area around Norway. Antibiotic sensitivity testing revealed resistance or reduced susceptibility to ampicillin (74%), oxolinic acid (33%), imipenem (21%), aztreonam (19%), and tobramycin (17%). Whole‐genome sequence analysis of eighteen drug‐resistant isolates revealed the presence of genes like β‐lactamases, chloramphenicol‐acetyltransferases, and genes conferring tetracycline and quinolone resistance. The strains also carried virulence genes like hlyA, tlh, rtxA to D and aceA, E and F. The genes for cholerae toxin (ctx), thermostable direct hemolysin (tdh), or zonula occludens toxin (zot) were not detected in any of the isolates. The present study shows low prevalence of multidrug resistance and absence of virulence genes of high global concern among environmental vibrios in Norway. However, in the light of climate change, and projected rising sea surface temperatures, even in the cold temperate areas, there is a need for frequent monitoring of resistance and virulence in vibrios to be prepared for future public health challenges.

中文翻译：

挪威海洋环境中的弧菌：相关抗生素耐药性和毒力基因的特征。

总共 116 个弧菌分离株，包括溶藻弧菌( n = 53)、梅斯尼科维弧菌( n = 38)、鳗弧菌( n = 21) 、古氏弧菌( n = 2) 和福建弧菌( n = 2) 是从挪威周围温带海洋和极地海洋区域的海水、鱼类或双壳类软体动物中获得的。抗生素敏感性测试显示对氨苄西林 (74%)、奥索林酸 (33%)、亚胺培南 (21%)、氨曲南 (19%) 和妥布霉素 (17%) 耐药或敏感性降低。对 18 种耐药分离株的全基因组序列分析揭示了 β-内酰胺酶、氯霉素乙酰转移酶等基因以及赋予四环素和喹诺酮耐药性的基因的存在。这些菌株还携带hly A、 tlh 、 rtx A 至 D 和ace A、E 和 F 等毒力基因。未检测到霍乱毒素 ( ctx )、耐热直接溶血素 ( tdh ) 或闭塞带毒素 ( zot ) 基因在任何分离株中。本研究表明，挪威环境弧菌的多重耐药性患病率较低，且不存在引起全球高度关注的毒力基因。然而，鉴于气候变化和预计海面温度上升，即使在寒温带地区，也需要经常监测弧菌的耐药性和毒力，为未来的公共卫生挑战做好准备。

更新日期：2020-06-17

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