A label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance (R_ct), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10⁻¹⁵–1.0×10⁻⁷ M) with a theoretical LOD of 3.01×10⁻¹⁶ M (3S/m). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.

中文翻译：

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使用基于3D还原的氧化石墨烯和聚苯胺纳米纤维的无标签电化学生物传感器对乳腺癌BRCA1生物标志物进行超灵敏检测。

基于三维还原氧化石墨烯（3D-rGO）和聚苯胺（PANI）纳米纤维修饰的玻碳电极（GCE）的无标签DNA生物传感器已成功开发用于乳腺癌BRCA1的超灵敏检测。结果表明3D-rGO和PANI纳米纤维具有降低电荷转移电阻（R _ct）的协同作用，这意味着3D-rGO-PANI / GCE的电化学活性大大增强。探针DNA可以固定在3D-rGO-PANI / GCE上，以对目标DNA进行特殊而灵敏的识别（1.0×10 ^-15 –1.0×10 ^-7  M），理论LOD为3.01×10 ^-16  M（3 S /米）。此外，这种拟议的纳米生物传感器可以直接检测真实血液样本中的BRCA1。