Dissecting Oligomeric States with Photoactivated Localization Microscopy: A Numerical Model.,Cytometry Part A

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Dissecting Oligomeric States with Photoactivated Localization Microscopy: A Numerical Model.
Cytometry Part A ( IF 2.5 ) Pub Date : 2020-06-18 , DOI: 10.1002/cyto.a.24167
Brian Daniels ₁ , Christian Wunder ₂ , Vanessa Chen ₃ , Malte Renz ₃

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Although photoactivated localization microscopy offers the potential to interrogate protein interactions in the physiological environment of a cell, uncertainties in the detection efficiency of photoactivatable proteins lead to complications with data interpretation. Here, we present a numerical model that provides probabilities to detect neighboring molecules dependent on their oligomerization status, density, detection efficiency, and radius, and can be used to assess oligomeric states or detection efficiencies of two molecular species. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

中文翻译：

用光活化定位显微镜剖析寡聚态：数值模型。

尽管光活化定位显微镜提供了在细胞生理环境中询问蛋白质相互作用的潜力，但光活化蛋白质检测效率的不确定性会导致数据解释的复杂化。在这里，我们提出了一个数值模型，该模型提供检测邻近分子的概率，取决于它们的寡聚化状态、密度、检测效率和半径，并可用于评估两种分子种类的寡聚状态或检测效率。© 2020 作者。Cytometry Part A由 Wiley Periodicals, Inc. 代表 International Society for Advancement of Cytometry 出版。

更新日期：2020-06-18

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