A chemiluminescence (CL) assay on the basis of the tremendous enhancing effect of copper and nitrogen co-doped carbon dots (Cu,N-CDs) on the luminol–NaIO₄ reaction was introduced for the determination of nanomolar levels of 4-nitrophenol (4-NP). Cu,N-CDs were synthesized by a hydrothermal approach and characterized by TEM, XRD, EDX, and FTIR analysis. The potential CL mechanism was elucidated by recording the CL spectrum and by evaluating the influence of reactive oxygen species. It was found that 4-NP remarkably inhibited the luminol–NaIO₄–Cu,N-CDs reaction and reduced the CL signal of the reaction. This fact was applied for developing a CL assay for 4-NP. Under the optimized conditions, 4-NP could be determined in the concentration range of 0.25 to 150 nM, with a detection limit as low as 0.06 nM. This assay was successfully exploited for the analysis of 4-NP in real environmental samples.

基于铜和氮共掺杂碳点（Cu，N-CDs）对luminol–NaIO ₄反应的巨大增强作用，进行了化学发光（CL）测定，用于测定纳摩尔浓度的4-硝基苯酚（ 4-NP）。通过水热法合成了Cu，N-CDs，并通过TEM，XRD，EDX和FTIR分析对其进行了表征。通过记录CL光谱和评估活性氧的影响，阐明了潜在的CL机制。发现4-NP显着抑制了鲁米诺-NaIO ₄-Cu，N-CDs反应，降低了反应的CL信号。该事实被用于开发4-NP的CL测定。在最佳条件下，可以在0.25至150 nM的浓度范围内测定4-NP，检测限低至0.06 nM。该测定法已成功地用于分析实际环境样品中的4-NP。