A bis (thiosemicarbazone) based probe has been synthesized and structurally characterized. The probe exhibits good selectivity towards Zn(II), Cd(II) and Hg(II) ions in an aqueous solution containing 95% water with ratiometric fluorescence changes. The modes of coordination of the probe with these metal ions and binding properties have been examined using different spectral techniques. The binding constants, determined using fluorescence titration data, are found to be 9.8 × 10³, 1.39 × 10⁵ and 2.03 × 10¹³ M⁻¹, respectively for Zn(II), Cd(II) and Hg(II) complexes. The high sensitivity of the probe has been demonstrated by the very low limit of detection i.e. 5.1, 3.4 and 0.51 μM for Zn(II), Cd(II) and Hg(II) ions, respectively. Different coordination mode of these metal ions with the probe has resulted in varying intra-ligand fluorescence (λ_em nm, Zn(II): 488, Cd(II): 470 and Hg(II): 578) among these metal complexes.

已经合成了基于双（硫代半脲）的探针，并对其结构进行了表征。在含95％水的水溶液中，该探针对Zn（II），Cd（II）和Hg（II）离子具有良好的选择性，并且荧光比例发生了变化。已使用不同的光谱技术检查了探针与这些金属离子的配位模式和结合特性。的结合常数，使用荧光滴定数据来确定，被发现是9.8×10 ³，1.39×10 ⁵和2.03×10 ¹³中号^-1分别用于Zn（II），Cd（II）和Hg（II）配合物。探针的高灵敏度已通过极低的检测限得到了证明，分别为Zn（II），Cd（II）和Hg（II）离子分别为5.1、3.4和0.51μM。这些金属离子与探针的不同配位模式导致这些金属配合物中的配体内荧光发生变化（_λem nm，Zn（II）：488，Cd（II）：470和Hg（II）：578）。