Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 – 10.0 wt%) and ILs (0.1 – 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex]⁻ > [But]⁻ > [Pro]⁻ > [Ac]⁻ > Cl⁻. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Pro]) are most efficient in the partition of commercial CCM into polymeric micelles (PMs)-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values ≥ 25; purification yields ≥ 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules’ partition.

配制了由Pluronic F68，三嵌段两亲共聚物和基于胆碱的离子液体（ILs）组成的新型水相两相胶束系统（ATPMS），并将其用于姜黄素（CCM）的分离/纯化。还评估了在ATPMS组分存在下的CCM稳定性。在共聚物（1.0 – 10.0 wt％）和ILs（0.1 – 3.0 M）水溶液中，CCM稳定长达24小时。通过在pH 6.0溶液中向Pluronic F68 + McIlvaine缓冲液中添加胆碱ILs，可以实现非常温和的相分离条件（接近室温）。浊点温度的降低是依赖于IL阴离子的相对疏水性，[己烷] ^- >（但是）^- > [专业] ^- > [AC] ^- >氯^-。由更多疏水性IL（[Ch] [Hex]> [Ch] [But]> [Ch] [Pro]）组成的ATPMS在将商业CCM分配到富含聚合物胶束（PMs）的相中最有效。然后使用最佳的ATPMS（基于0.70 M [Ch] [But]和0.60 M [Ch] [Hex]的ATPMS）从姜黄的粗提物中纯化CCM 。两个系统对从蛋白中分离CCM的选择性非常高污染物（选择性值≥25；纯化产率≥12倍）。Pluronic基于F68的ATPMS通过使用基于胆碱的ILs作为佐剂来调节相分离温度和生物分子的分配，有望用于疏水性生物分子的选择性分离。