Rising tropospheric ozone is a major threat to the crops in the present climate change scenario. To investigate the EDU induced changes in proteins, two varieties of maize, the SHM3031 and the PEHM5, (hereafter S and P respectively) were treated with three EDU applications (0 = control, 50 and 200 ppm) (hereafter 0 = A, 1 and 2 respectively) (SA, S1, S2, PA, P1, P2 cultivar X treatments). Data on the morpho-physiology, enzymatic activity, and protein expression (for the first time) were collected at the vegetative (V, 45 DAG) and flowering (F, 75 DAG) developmental stages. The tropospheric ozone was around 53 ppb enough to cause phytotoxic effects. Protective effects of EDU were recorded in morpho-physiologically and biochemically. SOD, CAT and APX together with GR performed better under EDU protection in SHM3031 variety than PEHM5. The protein expression patterns in SHM3031 at the vegetative stage (28% proteins were increased, 7% were decreased), and at the flowering stage (17% increased, 8% decreased) were found. In PEHM5, a 14% increase and an 18% decrease (vegetative stage) whereas a 16% increase and a 20% decrease (flowering stage) were recorded in protein expression. Some protein functional categories, for instance, photosynthesis, carbon metabolism, energy metabolism, and defense were influenced by EDU. Rubisco expression was increased in SHM3031 whereas differentially expressed in PEHM5. Germin like protein, APX, SOD, and harpin binding proteins have enhanced defense regulatory mechanisms under EDU treatment during prevailing high tropospheric O3. The present study showed EDU protective roles in C4 plants as proven in C3.

在当前的气候变化情景中，对流层臭氧的上升是对作物的主要威胁。为了研究EDU诱导的蛋白质变化，对玉米两个品种SHM3031和PEHM5（以下分别称为S和P）进行了3次EDU施用（0 =对照，50和200 ppm）（以下0 = A，1和2）（SA，S1，S2，PA，P1，P2品种X处理）。在营养发育期（V，45 DAG）和开花发育期（F，75 DAG）收集有关形态生理，酶活性和蛋白质表达的数据（首次）。对流层臭氧约为53 ppb，足以引起植物毒性作用。在形态，生理和生化方面记录了EDU的保护作用。在SHM3031品种的EDU保护下，SOD，CAT和APX以及GR的表现要好于PEHM5。发现SHM3031在营养阶段的蛋白质表达模式（蛋白质增加了28％，减少了7％），在开花阶段（增加了17％，减少了8％）。在PEHM5中，蛋白质表达记录到增加了14％，减少了18％（营养期），而增加了16％，减少了20％（开花期）。EDU影响了某些蛋白质功能类别，例如光合作用，碳代谢，能量代谢和防御。在SHM3031中Rubisco表达增加，而在PEHM5中差异表达。在普遍的高对流层O3中，在EDU处理下，胚芽蛋白，蛋白质，APX，SOD和harpin结合蛋白具有增强的防御调节机制。本研究表明EDU在C4植物中的保护作用已在C3中得到证明。