Abstract We assessed the interference of a complex food microbiota, present in Minas artisanal cheese (MAC) produced in the Serro region, on the performance of enumeration protocols for lactic acid bacteria (LAB) and staphylococci. Initially, 140 isolates were obtained from 6 Serro MAC samples and plated for enumeration of LAB (3 variations of MRS, M17 and Petrifilm™ LAB) and staphylococci (Baird–Parker and Petrifilm™ STX); some target isolates did not grow or present atypical morphologies in their selective protocols. Then, Serro MAC samples (n = 55) were obtained and plated for LAB and staphylococci based on the same protocols: 2569 isolates were obtained from LAB and 1164 from staphylococci protocols: Petrifim™ LAB presented the highest frequencies of LAB-confirmed colonies, as Petrifilm™ STX for staphylococci (p

中文翻译：

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手工奶酪的复杂微生物群会干扰乳酸菌和葡萄球菌计数方案的性能

摘要 我们评估了在 Serro 地区生产的 Minas 手工奶酪 (MAC) 中存在的复杂食物微生物群对乳酸菌 (LAB) 和葡萄球菌计数协议性能的干扰。最初，从 6 个 Serro MAC 样品中获得了 140 个分离株，并进行了接种以计数 LAB（MRS、M17 和 Petrifilm™ LAB 的 3 种变体）和葡萄球菌（Baird–Parker 和 Petrifilm™ STX）；一些目标分离株在其选择性方案中没有生长或呈现非典型形态。然后，获得 Serro MAC 样品 (n = 55) 并根据相同的方案接种 LAB 和葡萄球菌：从 LAB 中获得 2569 个分离物，从葡萄球菌中获得 1164 个分离物：Petrifim™ LAB 呈现出最高频率的 LAB 确认菌落，如Petrifilm™ STX 用于葡萄球菌 (p