Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically. Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties. Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength. HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo. In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs). The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds. Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2. In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery. Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.

一些传统的中药（TCM）已被用于骨修复，但是，羟基红花黄A（HYSA）是一种典型的能促进中药循环的红花成分，在骨科领域中，临床上对骨生成和血管生成的研究很少。 。本文中，我们假设推测通过3D打印技术携带HYSA的合成生物活性玻璃（BG，1393）支架可以增强成骨修复特性。值得注意的是，涂覆壳聚糖/海藻酸钠的支架具有优异的药物控制释放能力，并显着提高了BG机械强度。通过涂覆壳聚糖/海藻酸钠薄膜将HYSA加载到BG支架中，并在体内和体外评估HYSA /支架的成骨性和血管生成。体外细胞培养结果显示，高剂量的HYSA（0.5 mg / ml）支架可促进骨髓基质细胞（rBMSCs）的增殖以及人脐静脉内皮细胞（HUVECs）的迁移，小管形成。高剂量的HYSA /支架也可以改善rBMSC的活性碱性磷酸酶（ALP）。qRT-PCR和Western blot结果表明，高剂量的HYSA /支架可以上调ALP，OCN，OPN和RUNX-2的表达以及HIF-1α和BMP-2的相对蛋白分泌。在动物实验中，在手术后8周，高剂量的HYSA /支架比未掺杂的支架具有更好的促进新骨形成的能力。从而，