The sharp increase in e-waste derived from great consumption of electronic products has become a potential global environmental challenge. Limited information is available about the potential detrimental impact of e-waste on aquatic organisms. The present study investigated the expression of detoxification-related genes and life-history parameter changes in Daphnia magna exposed to e-waste leachate, simultaneously integrating with the chemical analysis of typical pollutants from e-waste leachate. The study aims at assessing impacts of e-waste to aquatic invertebrates and providing insights into its toxic mechanisms. The results showed high concentration of heavy metals like Cu (1657.14 ± 259.3 μg g⁻¹, DW) and persistent organic compounds like polybrominated diphenyl ethers (7831.32 ± 1273.86 ng g⁻¹, DW) in stream sediments near e-waste dismantling areas. Chronic exposure to these pollutants can affect the growth and reproduction of D. magna, resulting in significant development retardation, decreased total egg production per female, and even smaller body size. Expression of some detoxification and reproduction-related genes including DappuHR96, CYP360a, P-gp, EcR, CYP314 and Vtg exhibited different response patterns depending on the e-waste leachate concentration. E-waste leachate may affect the expression of detoxification-related and growth and reproduction-related genes and disrupt the growth and reproduction processes of D. magna.

电子产品的大量消费导致电子废物的急剧增加已成为潜在的全球环境挑战。关于电子废物对水生生物的潜在有害影响的信息有限。本研究调查了与电子垃圾渗滤液接触的大型蚤（Daphnia magna）中与排毒相关的基因的表达和生活史参数的变化，同时结合了电子垃圾渗滤液中典型污染物的化学分析。该研究旨在评估电子废物对水生无脊椎动物的影响，并提供有关其毒性机制的见解。结果表明高浓度的重金属如Cu（1657.14±259.3μgg ^-1，DW）和持久性有机化合物，如电子废物拆解区附近的河流沉积物中的多溴二苯醚（7831.32±1273.86 ng g ^-1，DW）。长期暴露于这些污染物中会影响D. magna的生长和繁殖，从而导致发育迟缓，每只雌性产卵总量下降，甚至体型变小。一些解毒和再现相关的基因，包括表达DappuHR96，CYP360a，P-gp的，EcR的，CYP314和的VTG表现出不同的响应模式，具体取决于电子垃圾渗滤液的浓度。电子垃圾渗滤液可能会影响排毒相关基因以及生长与繁殖相关基因的表达，并扰乱D. magna的生长与繁殖过程。