Abstract Background Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Due to the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of salep on bifidobacterial growth by measuring the highest optical density (OD), calculating the specific growth rates, and determining the production of lactic acid and short-chain fatty acids (acetic, propionic and butyric acid) as result of bacterial fermentation. Result The OD and pH values obtained in this study showed that salep was utilized as a source of assimilable carbon and energy by the Bifidobacterium species. All Bifidobacterium strains produced lactic, acetic, propionic and butyric acid, indicating that salep is readily fermented by these bacteria. Salep at 1% (w/v) showed a similar effect on bifidobacterial growth as that promoted by 1% (w/v) glucose used as a traditional carbon source. Conclusion Bifidobacterium species can develop in media containing salep as well as in glucose and exhibit the potential to be used as new sources of prebiotics.

中文翻译：

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在 Salep 存在下双歧杆菌属的短链脂肪酸生产

摘要背景 Salep 是通过研磨干燥的兰花块茎获得的，用作食品工业中的一种有价值的成分。由于 salep 的葡甘露聚糖含量，它被认为具有益生元潜力。然而，关于 salep 的发酵特性和潜在益生元特性的研究信息很少。本研究的目的是通过测量最高光密度 (OD)、计算特定生长速率以及确定乳酸和短链脂肪酸（乙酸、丙酸和丁酸）的产量来研究 salep 对双歧杆菌生长的影响。酸）作为细菌发酵的结果。结果 本研究中获得的 OD 和 pH 值表明，双歧杆菌属利用 salep 作为可同化碳和能量的来源。所有双歧杆菌菌株都产生乳酸、乙酸、丙酸和丁酸，表明 salep 很容易被这些细菌发酵。1% (w/v) 的 Salep 对双歧杆菌生长的影响与用作传统碳源的 1% (w/v) 葡萄糖促进的效果相似。结论 双歧杆菌可在含有 salep 和葡萄糖的培养基中发育，并表现出用作益生元新来源的潜力。