Adipose tissue is important for the regulation of energy balance through its metabolic, cellular, and endocrine functions. Furthermore, the excessive storage of subcutaneous fat can seriously affect the health and carcass traits of domestic animals. Stromal vascular fraction (SVF) cell adipogenic differentiation increases the number of differentiated adipocytes and plays a role in lipid deposition. The adipogenic differentiation of SVF cells is regulated by various factors, including microRNAs and cytokines. Sirt6 and miR-33a are known to be involved in metabolism and adipogenesis, respectively; however, their effects on the adipogenic differentiation of ovine SVF cells were previously unknown. Thus, the aim of this study was to investigate this. The results showed that SIRT6 is a binding target for miR-33a. Moreover, overexpression or inhibition of miR-33a was found to change the expression of SIRT6 messenger RNA and protein. Furthermore, modulating SIRT6 altered the expression of adipogenic marker genes. In addition, miR-33a and SIRT6 were found to play opposing roles in adipogenesis. Specifically, we demonstrated that miR-33a is involved in the negative regulation of ovine SVF cell adipogenic differentiation by inhibiting the expression of SIRT6. These findings reveal a key role for miR-33a and SIRT6 in adipogenesis, which will enrich our understanding of the regulatory factors associated with SVF cell adipogenic differentiation and provide a basis for further study on this process.

脂肪组织通过其代谢、细胞和内分泌功能对能量平衡的调节很重要。此外，皮下脂肪的过度储存会严重影响家畜的健康和胴体性状。基质血管部分 (SVF) 细胞成脂分化增加了分化的脂肪细胞的数量并在脂质沉积中起作用。SVF 细胞的成脂分化受多种因素的调控，包括 microRNA 和细胞因子。已知 Sirt6和 miR-33a 分别参与代谢和脂肪生成；然而，它们对绵羊 SVF 细胞成脂分化的影响以前是未知的。因此，本研究的目的是对此进行调查。结果表明，SIRT6是 miR-33a 的结合靶点。此外，发现过表达或抑制 miR-33a 会改变 SIRT6 信使 RNA 和蛋白质的表达。此外，调节SIRT6改变了脂肪生成标记基因的表达。此外，发现 miR-33a 和 SIRT6 在脂肪生成中起相反的作用。具体而言，我们证明了 miR-33a 通过抑制SIRT6的表达参与了绵羊 SVF 细胞成脂分化的负调节。这些发现揭示了 miR-33a 和SIRT6在脂肪生成中的关键作用，这将丰富我们对与 SVF 细胞脂肪生成分化相关的调控因素的理解，并为进一步研究这一过程提供基础。