Stereochemical configuration of the drug is responsible for racemic switch with enantiomers in presence of chiral environment for human beings. Therefore, its determination in racemic and pharmaceutical samples becomes a challenge. Addressing this issue, an enantioselective electrochemical biomimetic sensor for discrimination of isomers of ethambutol (ETB) employing square wave voltammetry (SWV) is reported for the first time. For this purpose, a chiral host, β-Cyclodextrin based copper metal organic framework (CD-CuMOF) was synthesized and used for chelate complexation of ETB isomers (SS-ETB/RR-ETB). A glassy carbon electrode (GCE) is chemically modified using CD-CuMOF and carbon nanofibers (CNF) composite material to construct a sensor in the form of (CD-CuMOF-CNF-GCE). The behaviour of CD-CuMOF for ETB isomers on GCE is postulated to be an artificial enzyme model (AEM) as it mimics the catalytic activity similar to enzyme alcohol dehydrogenase for ETB. The biosensor exhibits excellent peak potential difference (ΔEp (SS-RR) = 108 mV) between ETB isomers using SWV showing a clear distinction in the racemic mixture. It showed a linear response in the range of 1.0 x 10^-7 to 1 x 10^-4 M and 5.0 x 10^-7 to 2.5 x 10^-4 M with low detection limit of 3.10 x 10^-8 M and 8.52 x 10^-8 M for RR-ETB and SS-ETB isomers respectively. The sensor was applied for the estimation of ETB isomers in racemic mixture and real samples viz., blood, urine and pharmaceutical. The CD-CuMOF is a low-cost material with higher stability than enzyme and offers an advantage for sensing and catalysis in future.

在人类手性环境中，药物的立体化学构型负责与对映异构体进行外消旋转换。因此，在外消旋和药物样品中的测定成为一个挑战。为了解决这个问题，首次报道了采用方波伏安法（SWV）鉴别乙胺丁醇（ETB）异构体的对映选择性电化学仿生传感器。为此目的，合成了基于β-环糊精的手性主体铜金属有机骨架（CD-CuMOF），并将其用于ETB异构体（SS-ETB / RR-ETB）的螯合络合。使用CD-CuMOF和碳纳米纤维（CNF）复合材料对玻璃碳电极（GCE）进行化学修饰，以构建（CD-CuMOF-CNF-GCE）形式的传感器。CD-CuMOF在GCE上对ETB异构体的行为被认为是一种人工酶模型（AEM），因为它模仿了类似于ETB的乙醇醇脱氢酶的催化活性。使用SWV的生物传感器在ETB异构体之间显示出极好的峰电位差（ΔEp（SS-RR）= 108 mV），显示出外消旋混合物的明显区别。它显示出1.0 x 10范围内的线性响应^-7至1 x 10 ^-4  M和5.0 x 10 ^-7至2.5 x 10 ^-4  M， RR-ETB和SS-ETB异构体的检测限分别为3.10 x 10 ^-8  M和8.52 x 10 ^-8 M 。该传感器用于估计外消旋混合物和实际样品（例如血液，尿液和药物）中的ETB异构体。CD-CuMOF是一种低成本材料，具有比酶更高的稳定性，并在将来提供传感和催化方面的优势。