Abstract Masson pine (Pinus massoniana Lamb) is a high-economic-value tree species in southern China. However, P. massoniana seedlings in nurseries are damaged by damping-off disease caused by Fusarium oxysporum. Although Trichoderma spp. are used to control pathogens, the biocontrol mechanism by which Trichoderma koningiopsis suppresses damping-off disease remains unclear. In the present study, T. koningiopsis inoculation significantly decreased damping-off symptoms in P. massoniana and promoted seedling growth. Physiological and biochemical measurements revealed that T. koningiopsis controlled F. oxysporum by reducing reactive oxygen species, lowering lipid peroxidation and cell death, increasing osmolyte content, stimulating the activities of antioxidant enzymes, and increasing soil fertility. Illumina MiSeq sequencing revealed that T. koningiopsis increased plant growth-promoting Acidobacteria (19.9%) and decreased F. oxysporum occurrence by 92.0%. In addition, plant growth-promoting rhizobacteria (Pseudomonas, Bradyrhizobium, and Enterobacter) and ectomycorrhizal fungi (Clavulina, Pezoloma, Sphaerosporella, and Tomentella) were significantly enriched in rhizosphere soil following T. koningiopsis treatment. Thus, T. koningiopsis is a potential biocontrol agent for damping-off disease caused by F. oxysporum that also promotes P. massoniana seedling growth.

中文翻译：

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Trichoderma koningiopsis 通过调节活性氧代谢、渗透势和根际微生物组来控制尖孢镰刀菌导致马尾松幼苗猝倒

摘要 马尾松（Pinus massoniana Lamb）是我国南方的高经济价值树种。然而，苗圃中的马尾松幼苗受到尖孢镰刀菌引起的猝倒病的损害。虽然木霉属。用于控制病原体，木霉抑制猝倒病的生物控制机制仍不清楚。在本研究中，T. koningiopsis 接种显着减少了马尾松的猝倒症状并促进了幼苗生长。生理和生化测量表明，T. koningiopsis 通过减少活性氧、降低脂质过氧化和细胞死亡、增加渗透物含量、刺激抗氧化酶的活性和增加土壤肥力来控制尖孢镰刀菌。Illumina MiSeq 测序显示 T. koningiopsis 增加了促进植物生长的酸杆菌 (19.9%)，并将尖孢镰刀菌的发生率降低了 92.0%。此外，植物生长促进根际细菌（假单胞菌、慢生根瘤菌和肠杆菌）和外生菌根真菌（棒状菌、Pezoloma、球孢子菌和毛球藻）在根际土壤中显着富集，在 T. koningiopsis 处理后。因此，T. koningiopsis 是一种潜在的生物防治剂，可用于抑制由尖孢镰刀菌引起的病害，同时还能促进马尾松幼苗的生长。和 Tomentella）在 T. koningiopsis 处理后在根际土壤中显着富集。因此，T. koningiopsis 是一种潜在的生物防治剂，可用于抑制由尖孢镰刀菌引起的病害，同时还能促进马尾松幼苗的生长。和 Tomentella）在 T. koningiopsis 处理后在根际土壤中显着富集。因此，T. koningiopsis 是一种潜在的生物防治剂，可用于抑制由尖孢镰刀菌引起的病害，同时还能促进马尾松幼苗的生长。