Abstract Sulfur nanoparticles (SNPs) were synthesized using elemental sulfur and sodium sulfide, capped with chitosan as a stabilizer (SNPES), and their properties were compared to SNPs prepared by acidification of sodium thiosulfate (SNPSTS). The SNPs were characterized using UV–visible spectroscopy, EDS, TEM, XRD, and TGA, and their antimicrobial activity was tested using the disk diffusion method and minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC) analysis. The SNPES showed a rod-shaped morphology with an average length of 87 nm, while SNPSTS exhibited a spherical shape with an average particle size of 17 nm. The rod-shaped SNPES showed higher thermal stability than the spherical SNPSTS. Both types of SNPs did not show significant antibacterial activity against Gram-negative (E. coli) bacteria but showed significant antibacterial activity against Gram-positive (L. monocytogenes) bacteria. Between the SNPs, SNPES showed higher growth-inhibiting activity against L. monocytogenes than SNPSTS.

中文翻译：

---

硫纳米粒子的抗菌活性：制备方法的影响

摘要 使用元素硫和硫化钠合成了硫纳米粒子 (SNP)，并以壳聚糖作为稳定剂 (SNPES) 封端，并将其性质与硫代硫酸钠酸化制备的 SNP (SNPSTS) 进行了比较。使用紫外可见光谱、EDS、TEM、XRD 和 TGA 对 SNP 进行表征，并使用圆盘扩散法和最低抑菌浓度 (MIC)/最低杀菌浓度 (MBC) 分析测试其抗菌活性。SNPES 呈棒状，平均长度为 87 nm，而 SNPSTS 呈球形，平均粒径为 17 nm。棒状 SNPES 显示出比球形 SNPSTS 更高的热稳定性。两种类型的 SNP 均未对革兰氏阴性菌（大肠杆菌）表现出显着的抗菌活性。大肠杆菌）细菌，但对革兰氏阳性（单核细胞增生李斯特氏菌）细菌显示出显着的抗菌活性。在 SNP 之间，SNPES 对单核细胞增生李斯特菌的生长抑制活性高于 SNPSTS。