Combining CeVO4 oxidase-mimetic catalysis with hexametaphosphate ion induced electrostatic aggregation for photometric sensing of alkaline phosphatase activity,Analytica Chimica Acta

当前位置： X-MOL 学术 › Anal. Chim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Combining CeVO4 oxidase-mimetic catalysis with hexametaphosphate ion induced electrostatic aggregation for photometric sensing of alkaline phosphatase activity
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.06.024
Kun Ye ₁ , Xiangheng Niu ₁ , Hongwei Song ₂ , Linjie Wang ₁ , Yinxian Peng ₃

Affiliation

In the present work, a novel alkaline phosphatase (ALP) activity colorimetric assay is proposed by integrating the oxidase-mimicking catalytic characteristic of CeVO4 nanoparticles with the hexametaphosphate ion (HMPi) mediated electrostatic aggregation. The CeVO4 nanoparticles exhibit good oxidase-mimetic catalytic ability to promote the 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to TMBox, offering a significant change from colorless to blue. After a small amount of HMPi is added, the strong electrostatic interaction between the negatively charged HMPi species and the positively charged TMBox product leads to the aggregation of the latter, generating an aubergine HMPi-TMBox agglomerate. After the agglomerate is filtered out, the reaction solution turns to be almost colorless. When ALP is used to hydrolyze the HMPi species in advance, the electrostatic aggregation process is remarkably restrained, thus retaining the blue color of the CeVO4 catalyzed TMB solution. According to the new sensing strategy, highly selective and sensitive analysis of ALP activity was realized, providing a wide detection range from 1 to 210 U/L and a detection limit of 0.68 U/L. Accurate measurement of ALP activity in clinical serum matrices was also validated, demonstrating the practicability of the proposed assay in practical applications.

中文翻译：

将 CeVO4 氧化酶模拟催化与六偏磷酸根离子诱导的静电聚集相结合，用于光度传感碱性磷酸酶活性

在目前的工作中，通过将 CeVO4 纳米颗粒的氧化酶模拟催化特性与六偏磷酸根离子 (HMPi) 介导的静电聚集相结合，提出了一种新的碱性磷酸酶 (ALP) 活性比色测定法。CeVO4 纳米颗粒表现出良好的氧化酶模拟催化能力，可促进 3,3',5,5'-四甲基联苯胺 (TMB) 氧化为 TMBox，从而提供从无色到蓝色的显着变化。添加少量 HMPi 后，带负电的 HMPi 物质与带正电的 TMBox 产物之间的强静电相互作用导致后者聚集，生成茄子 HMPi-TMBox 聚集体。滤出附聚物后，反应溶液变为几乎无色。当 ALP 用于预先水解 HMPi 物种时，静电聚集过程受到显着抑制，从而保留了 CeVO4 催化的 TMB 溶液的蓝色。根据新的传感策略，实现了对ALP活性的高选择性和高灵敏度分析，提供1-210 U/L的宽检测范围和0.68 U/L的检测限。还验证了临床血清基质中 ALP 活性的准确测量，证明了所提出的测定在实际应用中的实用性。

更新日期：2020-08-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11