Low fouling strategy of electrochemical biosensor based on chondroitin sulfate functionalized gold magnetic particle for voltammetric determination of Mycoplasma ovipneumonia in whole serum,Analytica Chimica Acta

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Low fouling strategy of electrochemical biosensor based on chondroitin sulfate functionalized gold magnetic particle for voltammetric determination of Mycoplasma ovipneumonia in whole serum
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.06.015
Shiyi Zhao ₁ , Yingxia Zhou ₁ , Lai Wei ₁ , Lihua Chen ₂

Affiliation

Based on the successful design and preparation of Fe3O4@Au@polyethylene glycol (PEG)@chondroitin sulfate (CS) nanoparticles, a novel electrochemical biosensor was elaborately developed for MO determination by the drop coating method. This biosensor demonstrates ultra-low fouling properties in complex biological systems, especially in whole serum. Only 2.40% of the differential pulse voltammetry (DPV) signal changes are shown in the resistance test of electrode interface to 100% goat serum. The constructed biosensor also presented a good selectivity (DNA samples for M1, M2 and M3give a response around 14.2-21.4%of that obtained by the target analyte), reproducibility (the relative standard deviation (RSD) is only 4.82%) and storage stability (93.2% of the initial response is maintained after 20 day). Moreover, this biosensor exhibits high analytical performance with a wide line arranges (10-17 M ∼ 10-12 M) in buffer, even in 100% goat serum. In both cases, a low limit of detection (LOD) of 3.3 aM(S/N = 3)is showed. To our knowledge, a few examples reported the application of electrochemical biosensors to the direct ultrahigh sensitive determination of MO, especially in whole serum.

中文翻译：

基于硫酸软骨素功能化金磁粉的电化学生物传感器伏安法测定全血清卵支原体的低污染策略

在成功设计和制备Fe3O4@Au@聚乙二醇（PEG）@硫酸软骨素（CS）纳米颗粒的基础上，精心研制了一种新型电化学生物传感器，用于滴涂法测定MO。这种生物传感器在复杂的生物系统中表现出超低污染特性，尤其是在全血清中。在电极界面对 100% 山羊血清的电阻测试中，只有 2.40% 的差分脉冲伏安法 (DPV) 信号变化显示。所构建的生物传感器还具有良好的选择性（M1、M2 和 M3 的 DNA 样品的响应约为目标分析物获得的响应的 14.2-21.4%）、重现性（相对标准偏差（RSD）仅为 4.82%）和储存稳定性（93.2% 的初始反应在 20 天后保持不变）。而且，该生物传感器具有很高的分析性能，即使在 100% 山羊血清中，缓冲液中的线排列也很宽（10-17 M ∼ 10-12 M）。在这两种情况下，都显示了 3.3 aM(S/N = 3) 的低检测限 (LOD)。据我们所知，一些例子报道了电化学生物传感器在直接超高灵敏度测定中的应用，尤其是在全血清中。

更新日期：2020-08-01

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