In spite of the fact that pesticides enhanced the quality and yield of the agricultural production however do have certain serious effects on the environment. This study was carried out for isolation and molecular identification of microorganisms from water for malathion biodegradation in aquatic system. PCR analysis was used for identification of the isolated fungus. The growth kinetics of A. flavus in the presence of malathion under different environmental factors (pH, temperature and malathion concentration) were evaluated. Furthermore, the degradation kinetics of malathion by A. flavus in aqueous media under different environmental factors was evaluated. The isolated microorganism was identified as A. flavus with respect to it relation to the data from the gene bank and the lowest nucleotide diversity value between the tested isolate and A. flavus. The identified isolate grew successfully in a media supplemented with malathion much faster than without it. Hundred percent of malathion initial concentration was degraded within 36 days of incubation with A. flavus. The temperature of 30 °C, pH value of 7 and malathion initial concentration of 5 mg/l were the optimum conditions of A. flavus for growth and degradation of malathion. Bioremediation of malathion residues in water using A. flavus isolate are promising and considered the first report.

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黄曲霉的分离与分子鉴定及其水中马拉硫磷生物降解潜力的研究

尽管农药提高了农业生产的质量和产量，但对环境确实有一定的严重影响。本研究旨在从水中分离和分子鉴定用于水生系统中马拉硫磷生物降解的微生物。PCR分析用于鉴定分离的真菌。评估了在不同环境因素（pH、温度和马拉硫磷浓度）下存在马拉硫磷的黄曲霉生长动力学。此外，还评估了不同环境因素下，黄曲霉在水性介质中对马拉硫磷的降解动力学。分离的微生物被鉴定为黄曲霉，因为它与来自基因库的数据有关，以及测试分离株和黄曲霉之间的最低核苷酸多样性值。黄曲霉。鉴定出的分离株在补充有马拉硫磷的培养基中成功生长比没有它快得多。10% 的马拉硫磷初始浓度在与黄曲霉孵育的 36 天内降解。温度30℃、pH值7、马拉硫磷初始浓度5mg/l是黄曲霉生长和降解马拉硫磷的最佳条件。使用黄曲霉分离物对水中马拉硫磷残留进行生物修复很有前景，被认为是第一份报告。用于马拉硫磷生长和降解的黄素。使用黄曲霉分离物对水中马拉硫磷残留进行生物修复很有前景，被认为是第一份报告。用于马拉硫磷生长和降解的黄素。使用黄曲霉分离物对水中马拉硫磷残留进行生物修复很有前景，被认为是第一份报告。