Agitated shoot cultures of two aronias, Aronia melanocarpa (Michx.) Elliott and Aronia arbutifolia (L.) Pers., were maintained on Murashige & Skoog medium (1 mg/l BA and 1 mg/l NAA), both with and without the addition of various biosynthetic precursors of phenolic acids and depsides (phenylalanine, cinnamic acid, benzoic acid and caffeic acid). Each substance was added in 5 concentrations (0.1–10 mmol/l), each concentration at two time points (at the beginning and on the 10th day of cultures). Twenty-four phenolic acids were determined in methanolic extracts of the biomasses collected after 20 days of growth cycles by means of HPLC method with DAD detection. The presence of seven compounds was confirmed in all the extracts—five depsides (neochlorogenic, chlorogenic, cryptochlorogenic, isochlorogenic and rosmarinic acids), and syringic and caffeic acids. The main metabolites in A. melanocarpa shoot extracts were isochlorogenic, chlorogenic and neochlorogenic acids (max. 249.88, 450.35, 192.16 mg/100 g DW). The main metabolites in A. arbutifolia shoot extracts were: chlorogenic, isochlorogenic and cryptochlorogenic acids (max. 361.60, 224.5, 526.2 mg/100 g DW). The largest total amounts of the compounds were confirmed in the cultures of both aronias after the addition of cinnamic acid (989.79 and 661.77 mg/100 g DW, respectively) and caffeic acid (854.99 and 1098.46 mg/100 g DW, respectively) at concentrations of 5 mmol/l on 10th day of growth cycles. These maximum amounts were 3.41, 3.42, 2.95 and 5.67 times higher, respectively, than in the control cultures. This is the first report documenting the high production of depsides in shoot cultures of black and red aronias after feeding with their biosynthetic precursors.

两个Aronias melanocarpa（Michx。）Elliott和Aronia arbutifolia的搅动苗芽培养物（L.）均在Murashige＆Skoog培养基（1 mg / l BA和1 mg / l NAA）上保持，无论是否添加酚酸和去酚肽（苯丙氨酸，肉桂酸，苯甲酸）的各种生物合成前体酸和咖啡酸）。每种物质均以5种浓度（0.1-10 mmol / l）添加，每种浓度在两个时间点（培养开始和第10天）添加。通过DAD检测的HPLC方法，在生长20天后收集的生物质的甲醇提取物中测定了24种酚酸。在所有提取物中均证实了七种化合物的存在-五种糊精（新绿原酸，绿原酸，隐绿原酸，异绿原酸和迷迭香酸）以及丁香酸和咖啡酸。黑色果皮中的主要代谢物芽提取物为异绿原酸，绿原酸和新绿原酸（最大249.88、450.35、192.16 mg / 100 g DW）。熊果曲霉的主要代谢产物芽提取物为：绿原酸，异绿原酸和隐绿原酸（最大361.60、224.5、526.2 mg / 100 g DW）。在浓度分别添加肉桂酸（分别为989.79和661.77 mg / 100 g DW）和咖啡酸（分别为854.99和1098.46 mg / 100 g DW）后，在两个无花果培养物中都确认到了最大量的化合物在生长周期的第10天达到5 mmol / l。这些最大量分别比对照培养物中高3.41、3.42、2.95和5.67倍。这是第一份报道了以黑色和红色无花果的生物合成前体为食的枝条培养物中高产糊精的报告。