Drynaria bonii H. Christ is a medicinal plant that has traditionally been used for the treatment of chronic diseases and tuberculosis. However, D. bonii has not yet been cultivated or propagated. In this study, we optimized the conditions for the spore culture of D. bonii to produce sporophytes. First, we evaluated the effects of various factors, such as temperature, light intensity, potting material, and pH, on spore germination and early gametophyte development. Under optimal culture conditions, 95–99% of the spores germinated within 2 weeks of culture. Next, based on these results, we established a culture system for gametophyte development and sporophyte production. Mature gametophytes first appeared 5 months post-germination (mpg), and at 12 mpg, the rate of production of heart-shaped gametophytes reached 56.6%. Sporophytes at the early first-leaf stage first appeared at 6 mpg, and at 12 mpg, the rate of formation of sporophytes reached 15.4%. Interestingly, 6.3% of all gametophytes produced multiple sporophytes. Additionally, 42–62% of the gametophytes also produced multiple secondary gametophytes, indicating a high potential of D. bonii gametophytes to generate new gametophytes and, subsequently, sporophytes. When transferred to new trays or pots, sporophytes grew well and showed 100% survival. Overall, we conclude that this spore culture system can be successfully used for the propagation of D. bonii sporophytes.

中文翻译：

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骨碎补孢子孢子培养：优化培养条件以及配子体和孢子体发育分析

Drynaria bonii H. Christ是传统上用于治疗慢性疾病和肺结核的药用植物。但是，邦尼梭菌尚未被培养或繁殖。在这项研究中，我们优化了D. bonii的孢子培养条件产生孢子体。首先，我们评估了各种因素对孢子萌发和早期配子体发育的影响，例如温度，光照强度，灌封材料和pH值。在最佳培养条件下，培养2周内有95–99％的孢子发芽。接下来，基于这些结果，我们建立了配子体发育和孢子体生产的培养系统。成熟的配子体首先在发芽后5个月出现（mpg），而在12 mpg时，心形配子体的产生率达到56.6％。第一叶初期的孢子体首先以6 mpg出现，而在12 mpg时，孢子体的形成率达到15.4％。有趣的是，所有配子体中有6.3％产生了多种孢子体。此外，42-62％的配子体也产生了多个次级配子体，D. bonii配子体产生新的配子体，继而产生孢子体。当转移到新的托盘或盆中时，孢子体生长良好，并显示出100％的存活率。总的来说，我们得出结论，该孢子培养系统可以成功地用于邦氏梭菌孢子体的繁殖。