The aim of the study was to assess the effects of silver nanoparticles (AgNPs1 = 2.7 d·nm, AgNPs2 = 6.5 d·nm) and silver nitrate (AgNO₃) on Enchytraeus crypticus and Folsomia candida using toxicity tests (OECD Guideline 220, 232). A 28-day chronic toxicity study was performed to evaluate the reproduction and mortality rate. E. crypticus reproduction was more sensitive to AgNO₃ with a 28dEC₅₀ of 86.40 (62.52–119.4) mg·kg⁻¹ dry weight (d.w.) compared to AgNPs1 (28dEC₅₀ = 119.3 (60.4–235.6) mg·kg⁻¹ d.w). Similarly, the reproduction of F. candida was inhibited the most by AgNO₃ with a 28dEC₅₀ of 126.2 (104.2–152.9) mg·kg⁻¹ d.w. followed by AgNPs1 (28dEC₅₀ = 158.7 (64.05–393.2) mg·kg⁻¹ d.w.) and AgNPs2 (28dEC₅₀ = 206.4 (181.9–234.1) mg·kg⁻¹ d.w.). No mortalities were observed for tested soil invertebrates exposed to AgNPs at concentrations up to 166 mg·kg⁻¹ d.w. of AgNPs1 and 300 mg·kg⁻¹ d.w. of AgNPs2, respectively. It was found that silver ions are more toxic in comparison with AgNPs.

中文翻译：

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银纳米颗粒和离子暴露对土壤无脊椎动物念珠菌和隐孢子虫的影响。

该研究的目的是使用毒性试验（OECD准则220、232）评估纳米银颗粒（AgNPs1 = 2.7 d·nm，AgNPs2 = 6.5 d·nm）和硝酸银（AgNO ₃）对隐球菌和念珠菌的影响。）。进行了为期28天的慢性毒性研究，以评估其繁殖和死亡率。E. crypticus再现是硝酸银更敏感₃与28dEC ₅₀的86.40（62.52-119.4）毫克公斤· ^-1干重（DW）相比AgNPs1（28dEC ₅₀  = 119.3（60.4-235.6）毫克公斤· ^-1 DW ）。同样，AgNO抑制念珠菌的繁殖₃的28dEC ₅₀为126.2（104.2–152.9）mg·kg ^-1 dw，随后是AgNPs1（28dEC ₅₀  = 158.7（64.05–393.2）mg·kg ^-1 dw）和AgNPs2（28dEC ₅₀  = 206.4（181.9–234.1） mg·kg ^-1 dw）。分别暴露于浓度高达166 mg·kg ^-1 dw AgNPs1和300 mg·kg ^-1 dw AgNPs2的被暴露于AgNPs的土壤无脊椎动物，没有观察到死亡率。发现银离子与AgNP相比更具毒性。