The profile of sirtuin 3 (SIRT3), 8-hydroxy-2′-deoxyguanosine (8-OHdG), brain-derived neurotrophic factor (BDNF) and serotonin (5-HT) in cord blood and in early breast milk was studied and it was related to perinatal factors. 5-HT and BDNF signalling systems have been claimed to play a critical role in intrauterine development, postnatal adaptation and lactation. Since prematurity and Caesarean birth are frequently associated with inflammation and related oxidative stress, an attempt was made to reveal the adaptive changes of the protective SIRT3 and the complex interplay among these bioactive components in cord blood and early breast milk. Three groups each consisting of 30 mothers were included in the study: mothers who underwent spontaneous vaginal birth at term (group I), Caesarean section at term (group II) and preterm birth (group III). Venous cord blood and early breast milk samples were collected for measuring the biomarkers. SIRT3, 8-OHdG, BDNF and 5-HT levels were determined by using commercially available ELISA kits. It was demonstrated that cord blood levels of SIRT3, BDNF and 5-HT were markedly reduced whereas those of 8-OHdG were significantly elevated after preterm birth when compared with birth at term. The Caesarean section was associated with a moderate decrease in BDNF and 5-HT, however, both SIRT3 and 8-OHdG remained unaffected. Breast milk levels of all biomarkers studied proved to be independent of their corresponding cord blood concentrations. In response to preterm birth breast milk SIRT3, 8-OHdG and 5-HT increased significantly, while a drastic fall occurred in BDNF. A significant positive relationship was found of 5-HT with SIRT3 and 8-OHdG irrespective of the gestational age and the mode of delivery. It is suggested that the selected biomarkers in the breast milk mostly derive from local production by the mammary glands and 5-HT may have an essential role in the control of this process.

中文翻译：

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围产期因素对脐带血和早期母乳中sirtuin 3、8-羟基-2'-脱氧鸟苷，脑源性神经营养因子和血清素的影响：一项观察性研究。

研究了脐带血和早期母乳中sirtuin 3（SIRT3），8-羟基-2'-脱氧鸟苷（8-OHdG），脑源性神经营养因子（BDNF）和5-羟色胺（5-HT）的分布，与围产期因素有关。5-HT和BDNF信号传导系统据称在子宫内发育，产后适应和泌乳中起关键作用。由于早产和剖腹产通常与炎症和相关的氧化应激相关，因此试图揭示保护性SIRT3的适应性变化以及脐带血和早期母乳中这些生物活性成分之间的复杂相互作用。该研究包括三组，每组由30名母亲组成：足月（I组）进行自发阴道分娩的母亲，足月（II组）进行剖腹产和早产（III组）的母亲。收集静脉血和早期母乳样品以测量生物标志物。SIRT3、8-OHdG，BDNF和5-HT水平通过使用市售ELISA试剂盒进行测定。结果表明，与足月分娩相比，早产后SIRT3，BDNF和5-HT的脐血水平显着降低，而8-OHdG的脐血水平显着升高。剖宫产与BDNF和5-HT的适度降低有关，但是SIRT3和8-OHdG均未受影响。事实证明，所有研究的生物标志物的母乳水平均与其相应的脐带血浓度无关。响应早产母乳，SIRT3、8-OHdG和5-HT显着增加，而BDNF急剧下降。无论孕龄和分娩方式如何，5-HT与SIRT3和8-OHdG均呈显着正相关。建议在母乳中选择的生物标记物主要来自乳腺的局部生产，而5-HT在控制该过程中可能起重要作用。