A selective, sensitive, and rapid method by using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of histamine in fish and fish sauce was developed. The optimal conditions of liquid chromatographic separation and mass spectroscopy of histamine have also been investigated. The linear ranges of the method were 20.0 ÷ 1000 ng/mL, and the corresponding correlation coefficient was 0.9993. Mean recoveries of the analyte at three spike levels (low, medium, and high) were within the range of 98.5% ÷ 102.5% (n = 7). The limit of detection (LOD) and limit of quantification (LOQ) values were 3.83 and 11.50 ng/mL for the fish sauce sample and 4.71 and 14.12 ng/mL for the fish sample, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the method was negligible. The recommended method was applied to determine the content of this substance in 21 fish sauce samples and 4 kinds of fish samples, which were collected from Ho Chi Minh City, Vietnam, in 2019.

中文翻译：

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UPLC-MS / MS建立高灵敏度高选择性测定越南鱼和鱼露中组胺的方法

建立了一种选择性，灵敏，快速的超高效液相色谱-串联质谱法（UPLC-MS / MS）测定鱼和鱼露中组胺的方法。还研究了液相色谱分离和组胺质谱的最佳条件。该方法的线性范围为20.0÷1000 ng / mL，相应的相关系数为0.9993。三个加标水平（低，中和高）的分析物平均回收率在98.5％÷102.5％（n = 7）。鱼露样品的检测极限（LOD）和定量极限（LOQ）值分别为3.83和11.50 ng / mL，鱼样品为4.71和14.12 ng / mL。基质效应对方法的准确性，可重复性和回收率的影响可以忽略不计。该推荐方法适用于测定2019年从越南胡志明市采集的21种鱼露样品和4种鱼样品中的这种物质的含量。