This paper reports a simple, sensitive and reliable method for the simultaneous detection of aflatoxin B1, B2, G1 and G2 in vegetable oils. Aflatoxins were extracted by magnetic solid phase extraction followed by high-performance liquid chromatography, then postcolumn photochemical derivatization and finally detected by fluorescence detector. Vegetable oil samples were first diluted with hexane and then commercial bare Fe₃O₄ nanoparticles were directly employed as sorbents to extract aflatoxins from complex vegetable oil samples, which significantly simplified the procedure of sample preparation and largely improved the sample analysis throughput. The effects of various parameters such as the amount of sorbent, loading, washing and eluting conditions were carefully optimized to improve the extraction efficiencies of aflatoxins. Under the optimal conditions, the limits of detection of four aflatoxins ranged from 0.01 μg/kg to 0.16 μg/kg, and squared regression coefficients (R²) >0.9990 were obtained within the linear range of 0.1–20 μg/kg (except for aflatoxin G2 with 0.5–20 μg/kg). Furthermore, the recoveries spiked at four concentration levels in a blank vegetable oil sample were from 82.6 to 106.2%, with inter- and intraday relative standard deviations <9.8%, indicating good accuracy and precision of the proposed method.

中文翻译：

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裸露的Fe3O4作为磁吸附剂结合高效液相色谱和荧光检测技术快速，灵敏地检测植物油中的黄曲霉毒素B1，B2，G1和G2。

本文报告了一种同时检测植物油中黄曲霉毒素B1，B2，G1和G2的简单，灵敏和可靠的方法。黄曲霉毒素先用磁性固相萃取，再用高效液相色谱法萃取，再进行柱后光化学衍生，最后用荧光检测器检测。首先用己烷稀释植物油样品，然后用市售的裸露Fe ₃ O ₄稀释。纳米颗粒直接用作吸附剂，从复杂的植物油样品中提取黄曲霉毒素，这大大简化了样品制备过程，并大大提高了样品分析通量。精心优化了各种参数的影响，例如吸附剂的量，上样量，洗涤和洗脱条件，以提高黄曲霉毒素的提取效率。在最佳条件下，四种黄曲霉毒素的检出限范围为0.01μg/ kg至0.16μg/ kg，并且回归系数平方（R ²）在0.1–20μg/ kg的线性范围内获得了> 0.9990（黄曲霉毒素G2的0.5–20μg/ kg除外）。此外，空白植物油样品中四个浓度水平的加标回收率从82.6％增至106.2％，日间和日间相对标准偏差<9.8％，表明该方法具有良好的准确性和精密度。