The two species of the Bokermannohyla claresignata species group (Anura: Hylidae) have not been collected for the last four decades. It is the only species group of the hyline tribe Cophomantini that has not yet been analysed genetically. Its phylogenetic position is thus uncertain, and it has a combination of adult and larval character states that make this group a crucial missing piece that hinders our understanding of Cophomantini phylogenetics and character evolution. We obtained DNA sequences from a museum larval specimen of Bok. claresignata, using specialized extraction methods and high-throughput DNA sequencing, and combined the molecular phylogenetic results with available phenotypic information to provide new insights into the taxonomy and phylogenetic relationships of its species group. Our phylogenetic results place Bok. claresignata as sister to the Boana pulchella group, supporting its inclusion in Boana, together with Bokermannohyla clepsydra. In light of this new finding, we recognize a newly defined Boana claresignata group to accommodate these species, thus resolving both the polyphyly of Bokermannohyla and the paraphyly of Boana. Considering the phylogenetic relationships of the Boana claresignata group, we also discuss the evolution of suctorial tadpoles and mature oocyte/egg pigmentation in Cophomantini.

中文翻译：

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博物馆标本的高通量 DNA 测序揭示了 Bokermannohyla claresignata 组（Anura：Hylidae：Cophomantini）中长期失踪的物种

Bokermannohyla claresignata 物种组（Anura：Hylidae）的两个物种在过去的四年里一直没有被收集到。它是 hyline 部落 Cophomantini 中唯一尚未进行遗传分析的物种组。因此，它的系统发育位置是不确定的，并且它具有成虫和幼虫特征状态的组合，这使得该组成为阻碍我们理解 Cophomantini 系统发育和特征进化的关键缺失部分。我们从博克的博物馆幼虫标本中获得了 DNA 序列。claresignata，使用专门的提取方法和高通量 DNA 测序，并将分子系统发育结果与可用的表型信息相结合，为其物种群的分类学和系统发育关系提供新的见解。我们的系统发育结果放置 Bok。claresignata 作为 Boana pulchella 集团的姐妹，支持其与 Bokermannohyla clepsydra 一起纳入 Boana。根据这一新发现，我们识别出一个新定义的 Boana claresignata 群来容纳这些物种，从而解决了 Bokermannohyla 的多系和 Boana 的并系。考虑到 Boana claresignata 组的系统发育关系，我们还讨论了 Cophomantini 中吸管蝌蚪和成熟卵母细胞/卵色素沉着的进化。