ABSTRACT An experiment was conducted to determine the ileal endogenous calcium (Ca) losses in four-week old male Ross 308 broiler chickens (mean body weight ± SD, 1.66 ± 0.12 kg) using three assay methodologies. Three experimental diets were used: a Ca and phosphorus (P)-free diet and two other diets by substituting (w/w) 200 g/kg dextrose (w/w) with maize gluten meal or dried egg albumen as Ca-free protein sources. Each of the three experimental diets was randomly allocated and fed for three days to six replicate cages (six birds/cage), and ileal digesta were collected to measure the ileal endogenous Ca losses. The feed intake of birds fed diets containing the two protein sources was higher (P < 0.05) than the Ca and P-free diet. Body weight loss was observed in birds on the Ca and P-free diet and maize gluten meal-based diets. Ileal endogenous Ca losses were influenced (P < 0.05) by the assay methodology. Ileal endogenous Ca losses were determined to be 125, 77 and 46 mg/kg dry matter intake in birds fed the Ca and P-free diet, maize gluten meal diet and dried egg albumen diet, respectively. Ileal endogenous P losses were determined in birds fed Ca and P-free and dried egg albumen based diets. There was no effect (P > 0.05) of methodology on ileal endogenous P losses.

中文翻译：

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方法学对肉鸡回肠内源性钙损失测量的影响

摘要 使用三种测定方法进行了一项实验，以确定 4 周龄雄性 Ross 308 肉鸡（平均体重 ± SD，1.66 ± 0.12 kg）的回肠内源性钙 (Ca) 损失。使用了三种实验日粮：一种不含钙和磷 (P) 的日粮，另两种日粮通过用玉米蛋白粉或干蛋清代替 (w/w) 200 g/kg 葡萄糖 (w/w) 作为不含钙的蛋白质来源。三种实验日粮中的每一种都随机分配给六个重复的笼子（六只鸟/笼子）饲喂三天，并收集回肠食糜以测量回肠内源性钙损失。饲喂含有两种蛋白质来源的日粮的鸟类的采食量高于不含钙和磷的日粮（P < 0.05）。在无钙和无磷饮食和以玉米面筋粉为基础的饮食的鸟类中观察到体重减轻。回肠内源性 Ca 损失受测定方法的影响 (P < 0.05)。在饲喂无钙和无磷日粮、玉米面筋粉日粮和干蛋清日粮的鸟类中，回肠内源性钙损失分别测定为 125、77 和 46 mg/kg 干物质摄入量。在饲喂无钙和无磷以及基于干蛋清的饮食的鸟类中测定回肠内源性磷损失。方法学对回肠内源性 P 损失没有影响 (P > 0.05)。