Abstract Large-scale production of thiocyanate (SCN-) for industrial purposes has led to widespread environmental pollution by this compound. Thiocyanate (SCN-) is toxic for humans and recalcitrant to biological oxygenation and hydrolysis. Phytoremediation is an effective technique for disposal of SCN- because thiocyanate (SCN-) can be assimilated by many plants from contaminated water and soil. In this study, we demonstrate that Thermosynechococcus elongatus BP-1 glutathione S-transferase (TeGST) is a good candidate gene for enhancing phytoremediation of thiocyanate (SCN-) in the plant. Our study demonstrated that Arabidopsis thaliana introduced with the glutathione S-transferase (GST) gene were able to germinate and grow in a medium containing 5 mmol L −1 thiocyanate (SCN-), which was lethal for wild-type plants. Moreover, the GST can confer the capacity of plants to remove more thiocyanate in vivo. Our results suggest that grafting the T. elongatus BP-1 glutathione S-transferase (TeGST) gene into plants is a potentially effective strategy to enhance phytoremediation of environmental thiocyanates. To our knowledge, this is the first study on the degradation of guanidine isothiocyanate by transgenic plants.

中文翻译：

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Thermosynechococcus elongatus BP-1谷胱甘肽S-转移酶在拟南芥中的重组表达：硫氰酸盐植物修复的有效工具

摘要 用于工业目的的硫氰酸盐 (SCN-) 的大规模生产导致该化合物对环境造成了广泛的污染。硫氰酸盐 (SCN-) 对人体有毒，对生物氧化和水解具有顽抗性。植物修复是处理 SCN- 的有效技术，因为硫氰酸盐 (SCN-) 可以被许多植物从受污染的水和土壤中吸收。在这项研究中，我们证明细长嗜热链球菌 BP-1 谷胱甘肽 S-转移酶 (TeGST) 是增强植物中硫氰酸盐 (SCN-) 植物修复的良好候选基因。我们的研究表明，引入谷胱甘肽 S-转移酶 (GST) 基因的拟南芥能够在含有 5 mmol L -1 硫氰酸盐 (SCN-) 的培养基中发芽和生长，这对野生型植物是致命的。而且，GST 可以赋予植物在体内去除更多硫氰酸盐的能力。我们的结果表明，将 T. elongatus BP-1 谷胱甘肽 S-转移酶 (TeGST) 基因嫁接到植物中是增强环境硫氰酸盐植物修复的潜在有效策略。据我们所知，这是转基因植物降解异硫氰酸胍的首次研究。