Abstract

Chlorella sorokiana CTT 7727 was cultivated and the accumulated lipids extracted using ultrasound and agitation combined with solvents. The yields of lipids increased with the polarity of the solvent. The obtained yields were 14–16% for a mixture of chloroform and methanol (2:1), 9–15% for methanol, and 5–13% for ethanol. Chloroform and hexane did not surpass 3% of the recovered lipids. Despite the polarity of the solvents affected the yields of lipids, fatty acids methyl ester (FAME) amounts and profiles were similar. C16:0, C16:1, C18:0, C18:1, C18:2 and C18:3 were the FAMEs present in higher contents. C. sorokiniana has predominantly polar lipids, which could be extracted using ethanol. The productivity of lipids extracted did not exceed 5 mg mL⁻¹ h⁻¹ for all methods. The FAMEs have the potential to be used as blends with other biodiesels to increase their properties.

摘要

培养小球藻CTT 7727 并使用超声波和搅拌结合溶剂提取积累的脂质。脂质的产量随着溶剂的极性而增加。氯仿和甲醇 (2:1) 的混合物的收率为 14-16%，甲醇为 9-15%，乙醇为 5-13%。氯仿和己烷不超过回收脂质的 3%。尽管溶剂的极性会影响脂质的产量，但脂肪酸甲酯 (FAME) 的量和分布是相似的。C16:0、C16:1、C18:0、C18:1、C18:2 和 C18:3 是含量较高的 FAME。C. sorokiniana主要具有极性脂质，可以使用乙醇提取。提取的脂质产率不超过 5 mg mL ^-1 h ^-1对于所有方法。FAME 有可能被用作与其他生物柴油的混合物以增加其性能。